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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-9-7
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pubmed:databankReference |
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pubmed:abstractText |
Dexamethasone addition to cultured hepatocytes caused a 90-fold increase in mRNA for 6-phosphofructo 2-kinase/fructose-2,6-bisphosphatase. Glucocorticoid administration in vivo also increased the enzyme's mRNA in skeletal muscle by 3-4-fold. The sequence of the 5'-flanking region of the enzyme's gene revealed at least one consensus glucocorticoid response element. The amino acid sequence derived from a partial cDNA clone for the rat skeletal muscle bifunctional enzyme was identical to that of the liver isozyme except for an undetermined amount of N-terminal sequence. It is concluded that the rat muscle and liver isozymes, which are postulated to be identical except for the N-terminal region, are both regulated by glucocorticoids.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
753-60
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2547373-Animals,
pubmed-meshheading:2547373-Base Sequence,
pubmed-meshheading:2547373-Cells, Cultured,
pubmed-meshheading:2547373-DNA,
pubmed-meshheading:2547373-Exons,
pubmed-meshheading:2547373-Gene Expression Regulation,
pubmed-meshheading:2547373-Introns,
pubmed-meshheading:2547373-Isoenzymes,
pubmed-meshheading:2547373-Liver,
pubmed-meshheading:2547373-Male,
pubmed-meshheading:2547373-Molecular Sequence Data,
pubmed-meshheading:2547373-Muscles,
pubmed-meshheading:2547373-Phosphofructokinase-2,
pubmed-meshheading:2547373-Phosphoric Monoester Hydrolases,
pubmed-meshheading:2547373-Phosphotransferases,
pubmed-meshheading:2547373-Promoter Regions, Genetic,
pubmed-meshheading:2547373-RNA, Messenger,
pubmed-meshheading:2547373-Rats,
pubmed-meshheading:2547373-Rats, Inbred Strains,
pubmed-meshheading:2547373-Transcription, Genetic
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pubmed:year |
1989
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pubmed:articleTitle |
Sequence of the 5'-flanking region of the rat 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase gene: regulation by glucocorticoids.
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pubmed:affiliation |
Department of Physiology and Biophysics, State University of New York, Stony Brook 11794.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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