Linked Life Data

2545879

Source:http://linkedlifedata.com/resource/pubmed/id/2545879

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-8-14
pubmed:abstractText
When neutrophils were stimulated with f-met-leu-phe (fMLP) or phorbol myristate acetate (PMA) chemiluminescence (CL) augmented with luminol, lucigenin or 7-dimethylamino-naphthalene-1,2-dicarbonic acid hydrazide (DMNH) showed a strong dependence on the pH of the medium. CL increased in an exponential fashion when pH was increased from 6.5-9.2. All three luminescent intermediaries showed this pH-dependency but luminol was affected most. Unstimulated CL showed a similar increase with pH. Also neutrophil superoxide anion production (in the cytochrome C reduction assay) increased with pH, but not to the same extent as CL. CL induced by xanthine-oxidase plus hypoxanthine increased likewise with pH, and that increase was more pronounced than what was observed from the same reaction in the cytochrome C assay. The lucigenin-amplified neutrophil CL showed the best correlation to the superoxide production from neutrophils. Thus, several effects underlie the variation of CL with pH but the pH dependency of the luminescent intermediaries contribute substantially, which must be considered.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0141-2760
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-102
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Effects of extracellular pH on neutrophil superoxide anion production, and chemiluminescence augmented with luminol, lucigenin or DMNH.
pubmed:affiliation
Department of Medicine 3, Karolinska Institute at Södersjukhuset, Stockholm, Sweden.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't