Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-8-23
pubmed:abstractText
A method of in situ hybridization using single-stranded RNA probes of opposite polarity for quantitative enumeration of hepatitis A virus (HAV) in infected cells has been developed. Kinetic experiments showed that foci of infected cells appeared as early as day 2 postinfection. The absence of foci in cells examined immediately after virus adsorption indicated that foci detected subsequently were related to viral replication. Foci were detected by hybridization with RNA probes complementary to HAV genomic RNA but not with RNA probes identical to HAV genomic RNA. The number of foci observed was linearly related to the HAV dose inoculated. Focus formation was reduced when a virus inoculum was pretreated with guinea pig anti-HAV hyperimmune serum but not when it was pretreated with preimmune serum. The high resolution of hybridization signals and relative rapidity of the test indicated that this technique will be useful for measuring serum neutralizing antibodies and for quantitative assay of infectious HAV.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-2447830, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-2829721, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-3005635, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-3022645, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-3029972, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-3035078, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-3035079, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-6086708, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-6260685, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-6306048, http://linkedlifedata.com/resource/pubmed/commentcorrection/2545742-6310601
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
874-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
In situ hybridization for quantitative assay of infectious hepatitis A virus.
pubmed:affiliation
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.