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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1989-7-6
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pubmed:abstractText |
Mice infected subcutaneously with wild-type Salmonella typhimurium, SR11, developed a significant splenomegaly when compared with mice infected with an equal number of a plasmid-cured strain. Further, the bacterial load in the spleen at 14 days after infection, measured as colony-forming units per gram tissue, was significantly higher in mice infected with the parent strain than in mice infected with the plasmid-cured strain. These data confirm the previously reported plasmid-associated ability of Salmonella to multiply within the spleen. In addition, lymph node cells (LNC) from mice infected with the parent strain had a significantly reduced ability to proliferate in response to concanavalin A, a T-cell mitogen, and to heat-killed S. typhimurium cells when compared with LNC isolated from mice infected with the plasmid-cured strain. Finally, reintroduction of a functional Tn5-tagged 90-kb plasmid into a plasmid-free strain restored its capacity to cause a marked splenomegaly and to suppress lymph node cell proliferation in BALB/c mice. These data demonstrate that the 90-kb plasmid of highly virulent S. typhimurium strains mediates several novel pathogenic properties in infected mice: (1) enhancement of the ability of Salmonella to multiply within the spleen; (2) stimulation of a splenic inflammatory response as displayed by marked splenomegaly; and (3) a general suppression of lymphocyte responsiveness to both T-cell mitogens and specific Salmonella antigens.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0147-619X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
48-58
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2543008-Animals,
pubmed-meshheading:2543008-Cells, Cultured,
pubmed-meshheading:2543008-DNA Transposable Elements,
pubmed-meshheading:2543008-Female,
pubmed-meshheading:2543008-Immunosuppression,
pubmed-meshheading:2543008-Lymphocyte Activation,
pubmed-meshheading:2543008-Lymphocytes,
pubmed-meshheading:2543008-Mice,
pubmed-meshheading:2543008-Mice, Inbred BALB C,
pubmed-meshheading:2543008-Plasmids,
pubmed-meshheading:2543008-Salmonella Infections,
pubmed-meshheading:2543008-Salmonella typhimurium,
pubmed-meshheading:2543008-Splenomegaly,
pubmed-meshheading:2543008-Virulence
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pubmed:year |
1989
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pubmed:articleTitle |
Novel virulence properties of the Salmonella typhimurium virulence-associated plasmid: immune suppression and stimulation of splenomegaly.
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pubmed:affiliation |
Department of Microbiology and Immunology, School of Medicine, College of Science and Mathematics, Wright State University, Dayton, Ohio 45431.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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