|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
|
|
pubmed:dateCreated |
1989-7-6
|
|
pubmed:abstractText |
CD43 (sialophorin, gpL115) is a sialoglycoprotein expressed on a wide variety of blood cells including lymphocytes, monocytes, neutrophils, and platelets. L10, an anti-CD43 mAb, has been shown to induce monocyte-dependent activation and proliferation of human T lymphocytes. We have studied the signaling mechanism involved in this activation process. Treatment of PBMC and purified populations of T cells and monocytes with L10 induced the hydrolysis of phosphoinositides with the resultant generation of the phosphoinositide-derived second messengers diacylglycerol and inositol phosphates. This was associated with the translocation of protein kinase C from cytosol to membrane fractions and an increase in free intracellular Ca2+ in treated cells. In human leukemic T cell lines, the magnitude of signaling via CD43 did not correlate with the density of the TCR/CD3 surface expression nor with the intensity of signaling via the TCR/CD3. Moreover, a mutant derived from the leukemic T cell line HPB-ALL that was severely defective in TCR/CD3 surface expression and signaling nevertheless had normal CD43 surface expression and signaling compared with the parent cell line. It is concluded that CD43 is functionally coupled to the phospholipase C/phosphoinositides signaling pathway. In human T cells, signaling via CD43 proceeds independently of TCR/CD3. The widespread expression of CD43 suggests a potentially important role for this molecule in orchestrating the activation of multiple cell types.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD43,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Spn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/UN1 sialoglycoprotein, human
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0022-1767
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
142
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4194-200
|
|
pubmed:dateRevised |
2011-9-7
|
|
pubmed:meshHeading |
pubmed-meshheading:2542404-Adult,
pubmed-meshheading:2542404-Animals,
pubmed-meshheading:2542404-Antibodies, Monoclonal,
pubmed-meshheading:2542404-Antigens, CD,
pubmed-meshheading:2542404-Antigens, CD3,
pubmed-meshheading:2542404-Antigens, CD43,
pubmed-meshheading:2542404-Antigens, Differentiation,
pubmed-meshheading:2542404-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2542404-Calcium,
pubmed-meshheading:2542404-Humans,
pubmed-meshheading:2542404-Lymphocyte Activation,
pubmed-meshheading:2542404-Mice,
pubmed-meshheading:2542404-Monocytes,
pubmed-meshheading:2542404-Phosphatidylinositols,
pubmed-meshheading:2542404-Receptors, Antigen, T-Cell,
pubmed-meshheading:2542404-Second Messenger Systems,
pubmed-meshheading:2542404-Sialoglycoproteins,
pubmed-meshheading:2542404-T-Lymphocytes
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
Mechanism of mononuclear cell activation by an anti-CD43 (sialophorin) agonistic antibody.
|
|
pubmed:affiliation |
Division of Allergy and Immunology, Children's Hospital, Boston, MA 02115.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
