|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1989-7-5
|
|
pubmed:abstractText |
Plasmid recombination, like other homologous recombination in Escherichia coli, requires RecA protein in most conditions. We have found that the plasmid recombination defect in a recA mutant can be efficiently suppressed by the beta protein of bacteriophage lambda. beta protein is required for homologous recombination of lambda chromosomes during lytic phage growth in a recA host and is known to have a strand-annealing activity resembling that of RecA protein. The bioluminescence recombination assay was used for genetic analysis of beta-protein-mediated plasmid recombination. Efficient suppression of the recA mutation by beta protein required the absence of the E. coli nucleases exonuclease I and RecBCD nuclease. These nucleases inhibit a RecA-mediated plasmid recombination pathway that is more efficient than the pathway functioning in wild-type cells. Like RecA-mediated plasmid recombination in RecBCD- ExoI- cells, beta-protein-mediated plasmid recombination depended on concurrent DNA replication and on the activity of the recQ gene. However, unlike RecA-mediated plasmid recombination, beta-protein-mediated recombination in RecBCD- ExoI- cells was independent of recF and recJ activities. We propose that inactivation of exonuclease I and RecBCD nuclease stabilizes a recombination intermediate that is involved in RecA- and beta-protein-catalyzed homologous pairing reactions. We suggest that the intermediate may be linear plasmid DNA with a protruding 3' end, since these nucleases are known to interfere with the synthesis of such linear forms. The different recF and recJ requirements for beta-protein-dependent and RecA-dependent recombinations imply that the mechanisms of formation or processing of the putative intermediate differ in the two cases.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-13475269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-150254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-156361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-22760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-2842780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-2940241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-2943636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-2948217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-2957271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-2958632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-2961561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-2982147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-2993230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-3005590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-3058984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-3157004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-3298213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-344137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-3522548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-368567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-370822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-379861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-3932331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-4205905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-4556461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-4568763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-4587405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-4598358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-4927675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-5338754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-5455134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-5760458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6244589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6259126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6273399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6326142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6343801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6346018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6363387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6374379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6381965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6759658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-6994103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-7029308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-7035834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542228-790387
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0021-9193
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
171
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3523-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2542228-Bacterial Proteins,
pubmed-meshheading:2542228-Bacteriophage lambda,
pubmed-meshheading:2542228-Escherichia coli,
pubmed-meshheading:2542228-Exodeoxyribonucleases,
pubmed-meshheading:2542228-Genes, Bacterial,
pubmed-meshheading:2542228-Luciferases,
pubmed-meshheading:2542228-Plasmids,
pubmed-meshheading:2542228-Rec A Recombinases,
pubmed-meshheading:2542228-Recombination, Genetic,
pubmed-meshheading:2542228-Suppression, Genetic,
pubmed-meshheading:2542228-Viral Proteins
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
Suppression of recA deficiency in plasmid recombination by bacteriophage lambda beta protein in RecBCD- ExoI- Escherichia coli cells.
|
|
pubmed:affiliation |
Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
