| pubmed-article:2540153 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2540153 | lifeskim:mentions | umls-concept:C0001699 | lld:lifeskim |
| pubmed-article:2540153 | lifeskim:mentions | umls-concept:C0017263 | lld:lifeskim |
| pubmed-article:2540153 | lifeskim:mentions | umls-concept:C1158467 | lld:lifeskim |
| pubmed-article:2540153 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2540153 | pubmed:dateCreated | 1989-6-7 | lld:pubmed |
| pubmed-article:2540153 | pubmed:abstractText | We isolated Mu dI1734 insertion mutants of Klebsiella pneumoniae that were unable to assimilate nitrate or nitrite as the sole nitrogen source during aerobic growth (Nas- phenotype). The mutants were not altered in respiratory (anaerobic) nitrate and nitrite reduction or in general nitrogen control. The mutations were linked and thus defined a single locus (nas) containing genes required for nitrate assimilation. beta-Galactosidase synthesis in nas+/phi(nas-lacZ) merodiploid strains was induced by nitrate or nitrite and was inhibited by exogenous ammonia or by anaerobiosis. beta-Galactosidase synthesis in phi(nas-lacZ) haploid (Nas-) strains was nearly constitutive during nitrogen-limited aerobic growth and uninducible during anaerobic growth. A general nitrogen control regulatory mutation (ntrB4) allowed nitrate induction of phi(nas-lacZ) expression during anaerobic growth. This and other results suggest that the apparent anaerobic inhibition of phi(nas-lacZ) expression was due to general nitrogen control, exerted in response to ammonia generated by anaerobic (respiratory) nitrate reduction. | lld:pubmed |
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| pubmed-article:2540153 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2540153 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2540153 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2540153 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2540153 | pubmed:month | May | lld:pubmed |
| pubmed-article:2540153 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:2540153 | pubmed:author | pubmed-author:StewardFF | lld:pubmed |
| pubmed-article:2540153 | pubmed:author | pubmed-author:CaliB MBM | lld:pubmed |
| pubmed-article:2540153 | pubmed:author | pubmed-author:MiccaJ LJL | lld:pubmed |
| pubmed-article:2540153 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2540153 | pubmed:volume | 171 | lld:pubmed |
| pubmed-article:2540153 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2540153 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2540153 | pubmed:pagination | 2666-72 | lld:pubmed |
| pubmed-article:2540153 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:2540153 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2540153 | pubmed:articleTitle | Genetic regulation of nitrate assimilation in Klebsiella pneumoniae M5al. | lld:pubmed |
| pubmed-article:2540153 | pubmed:affiliation | Department of Microbiology, Cornell University, Ithaca, New York 14853-7201. | lld:pubmed |
| pubmed-article:2540153 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2540153 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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