|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1989-6-7
|
|
pubmed:abstractText |
We isolated Mu dI1734 insertion mutants of Klebsiella pneumoniae that were unable to assimilate nitrate or nitrite as the sole nitrogen source during aerobic growth (Nas- phenotype). The mutants were not altered in respiratory (anaerobic) nitrate and nitrite reduction or in general nitrogen control. The mutations were linked and thus defined a single locus (nas) containing genes required for nitrate assimilation. beta-Galactosidase synthesis in nas+/phi(nas-lacZ) merodiploid strains was induced by nitrate or nitrite and was inhibited by exogenous ammonia or by anaerobiosis. beta-Galactosidase synthesis in phi(nas-lacZ) haploid (Nas-) strains was nearly constitutive during nitrogen-limited aerobic growth and uninducible during anaerobic growth. A general nitrogen control regulatory mutation (ntrB4) allowed nitrate induction of phi(nas-lacZ) expression during anaerobic growth. This and other results suggest that the apparent anaerobic inhibition of phi(nas-lacZ) expression was due to general nitrogen control, exerted in response to ammonia generated by anaerobic (respiratory) nitrate reduction.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-120727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-14104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-16079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-16262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-2832370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-2839825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-2867543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-2874557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-2999766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-3045516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-3301812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-338917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-342501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-37075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-41244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-4156359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-4399578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-4860579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-5085268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-5338848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-5600617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-5726295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6102551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6113591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6120441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6120931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6126518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6130464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6260375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6348020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6420393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6433849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6449399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6450746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6775047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-6789329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-7033208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-7047497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2540153-787532
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0021-9193
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
171
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2666-72
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2540153-Anaerobiosis,
pubmed-meshheading:2540153-Chlorates,
pubmed-meshheading:2540153-DNA Mutational Analysis,
pubmed-meshheading:2540153-DNA Transposable Elements,
pubmed-meshheading:2540153-Gene Expression Regulation,
pubmed-meshheading:2540153-Genes, Bacterial,
pubmed-meshheading:2540153-Genetic Linkage,
pubmed-meshheading:2540153-Klebsiella pneumoniae,
pubmed-meshheading:2540153-Multigene Family,
pubmed-meshheading:2540153-Nitrate Reductase,
pubmed-meshheading:2540153-Nitrate Reductases,
pubmed-meshheading:2540153-Nitrates,
pubmed-meshheading:2540153-Operon,
pubmed-meshheading:2540153-beta-Galactosidase
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
Genetic regulation of nitrate assimilation in Klebsiella pneumoniae M5al.
|
|
pubmed:affiliation |
Department of Microbiology, Cornell University, Ithaca, New York 14853-7201.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
