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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1989-6-2
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pubmed:abstractText |
Using flow-cytometric analysis and a fluorescein-conjugated anti-IgE antibody, we have investigated whether normal-density human eosinophils will bind greater amounts of native IgE following activation with platelet-activating factor (PAF), leukotriene B4 (LTB4) and histamine. Incubation with PAF resulted in a large dose- and time-dependent increase in IgE binding, optimal at 10(-7) M and 30 min, respectively. LTB4 and histamine gave lower but significant increases in IgE binding. Enhanced binding of IgE by activated normal-density eosinophils is probably a consequence of enhanced Fc epsilon RII receptor expression, suggesting a functional role for this receptor in allergic inflammation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0020-5915
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
194-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2540095-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:2540095-Eosinophils,
pubmed-meshheading:2540095-Flow Cytometry,
pubmed-meshheading:2540095-Histamine,
pubmed-meshheading:2540095-Humans,
pubmed-meshheading:2540095-Immunoglobulin E,
pubmed-meshheading:2540095-Leukotriene B4,
pubmed-meshheading:2540095-Platelet Activating Factor,
pubmed-meshheading:2540095-Receptors, Fc,
pubmed-meshheading:2540095-Receptors, IgE,
pubmed-meshheading:2540095-Time Factors
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pubmed:year |
1989
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pubmed:articleTitle |
Flow-cytometric analysis of increased IgE uptake by normal eosinophils following activation with PAF-acether and other inflammatory mediators.
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pubmed:affiliation |
Department of Allergy, National Children's Research Centre, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro
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