Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1989-5-18
|
| pubmed:abstractText |
Adult diarrhea rotavirus (ADRV) is a newly identified strain of noncultivable human group B rotavirus that has been epidemic in the People's Republic of China since 1982. We have used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western (immuno-) blot analysis to examine the viral proteins present in the outer and inner capsids of ADRV and compared these with the proteins of a group A rotavirus, SA11. EDTA treatment of double-shelled virions removed the outer capsid and resulted in the loss of three polypeptides of 64, 61, and 41, kilodaltons (kDa). Endo-beta-N-acetylglucosaminidase H digestion of double-shelled virions identified the 41-kDa polypeptide as a glycoprotein. CaCl2 treatment of single-shelled particles removed the inner capsid and resulted in the loss of one polypeptide with a molecular mass of 47 kDa. The remaining core particle had two major structural proteins of 136 and 113 kDa. All of the proteins visualized on sodium dodecyl sulfate-polyacrylamide gel electrophoresis were antigenic by Western blot analysis when probed with convalescent-phase human and animal antisera. A 47-kDa polypeptide was most abundant and was strongly immunoreactive with human sera, animal sera raised against ADRV and against other group B animal rotaviruses (infectious diarrhea of infant rat virus, bovine and porcine group B rotavirus, and bovine enteric syncytial virus) and a monoclonal antibody prepared against infectious diarrhea of infant rat virus. This 47-kDa inner capsid polypeptide contains a common group B antigen and is similar to the VP6 of the group A rotaviruses. Human convalescent-phase sera also responded to a 41-kDa polypeptide of the outer capsid that seems similar to the VP7 of group A rotavirus. Other polypeptides have been given tentative designations on the basis of similarities to the control preparation of SA11, including a 136-kDa polypeptide designated VP1, a 113-kDa polypeptide designated VP2, 64- and 61-kDa polypeptides designated VP5 and VP5a, and several proteins in the 110- to 72-kDa range that may be VP3, VP4, or related proteins. The lack of cross-reactivity on Western blots between antisera to group A versus group B rotaviruses confirmed that these viruses are antigenically quite distinct.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-229253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-2444622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-2460499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-2536755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-2835518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-2852892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-2984243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-3004767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-3009541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-3016112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-3021026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-3023498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-3036446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-6144874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-6245262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-6292454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-6297152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-6302311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-6307873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-6311947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2539512-6323312
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-538X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
63
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
2191-7
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:2539512-Adult,
pubmed-meshheading:2539512-Antigens, Viral,
pubmed-meshheading:2539512-Blotting, Western,
pubmed-meshheading:2539512-Capsid,
pubmed-meshheading:2539512-Diarrhea,
pubmed-meshheading:2539512-Glycoproteins,
pubmed-meshheading:2539512-Glycosylation,
pubmed-meshheading:2539512-Humans,
pubmed-meshheading:2539512-Molecular Weight,
pubmed-meshheading:2539512-Rotavirus,
pubmed-meshheading:2539512-Viral Proteins,
pubmed-meshheading:2539512-Viral Structural Proteins
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Purification and characterization of adult diarrhea rotavirus: identification of viral structural proteins.
|
| pubmed:affiliation |
Viral Gastroenteritis Unit, Centers for Disease Control, Atlanta, Georgia 30333.
|
| pubmed:publicationType |
Journal Article
|