| pubmed-article:2538309 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C0027836 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C0140080 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C0024742 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C0021665 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C0021666 | lld:lifeskim |
| pubmed-article:2538309 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:2538309 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:2538309 | pubmed:dateCreated | 1989-5-3 | lld:pubmed |
| pubmed-article:2538309 | pubmed:abstractText | We have used the rat C6 glial cell line as a model system to study the role of insulin-like growth factors (IGF) in neuroglial cells of the central nervous system (CNS). Northern blot analysis of C6 RNA demonstrated the presence of IGF-I mRNA and undetectable IGF-II mRNA. IGF-I and IGF-binding protein(s), but not IGF-II, were detected in C6 glial cell-conditioned medium. The level of IGF-I was 1-4 ng/ml in conditioned medium based on a human IGF-I standard. The immunoreactive IGF-I inhibited [125I]IGF-I binding to the IGF-I receptor on chick embryo fibroblasts and stimulated [3H]thymidine incorporation into chick embryo fibroblast DNA. Competitive binding and affinity cross-linking experiments using [125]IGF-I and [125I]IGF-II demonstrated the presence of IGF-I receptors (type I) and IGF-II/mannose 6-phosphate receptors (type II) on C6 glial cell membranes. An immunoglobulin (no. 3637) directed against the rat IGF-II receptor blocked the degradation of [125I]IGF-II added to C6 glial cells, presumably by blocking receptor-mediated internalization. We were unable to demonstrate an autocrine role for IGF in the C6 glial cell line, since [3H]thymidine incorporation into DNA was stimulated equally well by IGF-I-deficient rat serum and normal serum, and added IGF did not stimulate [3H]thymidine incorporation into DNA when tested alone or when added to IGF-I-deficient serum. We propose that neuroglial cell-derived IGF-I may serve as a paracrine growth stimulus in the central nervous system. | lld:pubmed |
| pubmed-article:2538309 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2538309 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2538309 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2538309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2538309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2538309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2538309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2538309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2538309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2538309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2538309 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2538309 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2538309 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:2538309 | pubmed:author | pubmed-author:NissleyS PSP | lld:pubmed |
| pubmed-article:2538309 | pubmed:author | pubmed-author:RechlerM MMM | lld:pubmed |
| pubmed-article:2538309 | pubmed:author | pubmed-author:LeeLL | lld:pubmed |
| pubmed-article:2538309 | pubmed:author | pubmed-author:GrahamD EDE | lld:pubmed |
| pubmed-article:2538309 | pubmed:author | pubmed-author:GreensteinLL | lld:pubmed |
| pubmed-article:2538309 | pubmed:author | pubmed-author:TsengL YLY | lld:pubmed |
| pubmed-article:2538309 | pubmed:author | pubmed-author:KiessWW | lld:pubmed |
| pubmed-article:2538309 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2538309 | pubmed:volume | 124 | lld:pubmed |
| pubmed-article:2538309 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2538309 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2538309 | pubmed:pagination | 1727-36 | lld:pubmed |
| pubmed-article:2538309 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:meshHeading | pubmed-meshheading:2538309-... | lld:pubmed |
| pubmed-article:2538309 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2538309 | pubmed:articleTitle | Rat C6 glial cells synthesize insulin-like growth factor I (IGF-I) and express IGF-I receptors and IGF-II/mannose 6-phosphate receptors. | lld:pubmed |
| pubmed-article:2538309 | pubmed:affiliation | Metabolism Branch, National Cancer Institute, Bethesda, Maryland 20892. | lld:pubmed |
| pubmed-article:2538309 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2538309 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2538309 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2538309 | lld:pubmed |
