Linked Life Data

2538121

Source:http://linkedlifedata.com/resource/pubmed/id/2538121

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-4-17
pubmed:abstractText
The methylation status of the 160 kD viral capsid antigen (VCA) gene promoter was determined by hybridization analysis. The semi-permissive marmoset cell line FF41-1 lacked cytosine methylation in approximately three quarters of the VCA promoter CpG dinucleotide residues. In the stringently infected HH514CL16 cell line the same CpG residues were methylated in three quarters of the genomes. 5'deoxy-5'-S-isobutyladenosine (SIBA), a DNA methylase inhibitor, was utilized to disrupt the EBV latent state. As determined by flow cytometry, SIBA treatment significantly increased expression of VCA. The VCA promoter was hypomethylated in VCA-positive FF41-1 cells sorted by flow cytometry. While hypomethylation alone was not sufficient for VCA transcriptional activity, the absence of methylation of VCA promoter CpG dinucleotide residues was associated with expression of VCA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-70
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Relationship between methylation status and expression of an Epstein-Barr virus (EBV) capsid antigen gene.
pubmed:affiliation
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.