Linked Life Data

2536773

Source:http://linkedlifedata.com/resource/pubmed/id/2536773

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-3-21
pubmed:abstractText
A persistent problem in the generation of antigen-specific human monoclonal antibodies is the rarity of appropriate B cells in human blood or splenic tissues. In order to immortalize the rare antigen-specific cells that are available, an electric field-induced cell fusion technique has been shown to markedly increase the fusion efficiency in comparison to polyethylene glycol-induced cell fusion using Epstein-Barr virus (EBV) or pokeweed mitogen activated B cells. Fusion efficiency of 10(-3)-10(-4) has been achieved by this process with as low as 1 X 10(6) input EBV-activated B cells. A panel of human monoclonal antibodies to human cytomegalovirus has subsequently been produced using this technique. This improvement should enable wider therapeutic and diagnostic applications of human monoclonal antibodies.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
117-22
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Electric field-induced cell fusion and human monoclonal antibodies.
pubmed:affiliation
Department of Pathology, Stanford University School of Medicine, CA 94305.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.