Linked Life Data

2536160

Source:http://linkedlifedata.com/resource/pubmed/id/2536160

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-2-14
pubmed:abstractText
The 5' flanking regions of the mouse renin genes (Ren1d and Ren2d) contain putative negative control and cAMP responsive elements. Sequence analysis shows additionally that these putative control elements in the Ren2d gene are interrupted by a 160-base-pair insertion. To document the functions of these elements, we isolated these regions and fused them to the reporter gene chloramphenicol acetyltransferase (CAT), which was linked upstream to a thymidine kinase (TK) promoter (pUTKAT1). The chimeric constructs were transfected into mouse pituitary tumor AtT-20 and human choriocarcinoma JEG-3 cells. At the basal unstimulated condition, Ren1d 5' flanking sequence in the sense orientation inhibited basal CAT expression from the TK promoter of pUTKAT1, whereas the same sequence in the antisense orientation did not. The 5' flanking region of Ren2d had no inhibitory effect on basal CAT expression. These data demonstrate that the negative control element is functional in Ren1d but is nonfunctional in Ren2d, suggesting that the 160-base-pair insertion in Ren2d interferes with the function of the negative control elements. In response to 8-bromo-cAMP, both renin genes increased transcription 3-fold, suggesting a functional cis action of the cAMP responsive element in both genes. These data may be important in the understanding of the regulation of the tissue-specific expression of mouse renin genes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-2875459, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-2893797, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-2991882, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3015903, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3020428, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3023200, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3040732, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3475989, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3530549, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3588288, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3665875, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3888409, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-3898081, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-6143318, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-6283362, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-6327270, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-6687701, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-6749879, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-6960240, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-7028030, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536160-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
56-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Negative control elements and cAMP responsive sequences in the tissue-specific expression of mouse renin genes.
pubmed:affiliation
Molecular and Cellular Vascular Research Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't