Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1992-10-22
|
| pubmed:abstractText |
We have used nuclear extracts prepared from tobacco leaf tissue to characterize a factor binding site, designated as-2 (activating sequence-2), at the -100 region of the cauliflower mosaic virus 35S promoter. The activity of this factor, called ASF-2 (activating sequence factor-2), is not detected in tobacco root extracts. as-2 includes two GT motifs with sequence homology to the SV40 enhancer core A element and the Box II element of pea rbcS. Nevertheless, oligomers of these sequence elements do not compete for ASF-2 binding in gel retardation assays, indicating that the GT motifs may not be involved. Methylation interference studies identify two guanines (G93 and G98) that are required for interaction with ASF-2. Sequences surrounding these two critical guanines display homologies to a GATA repeat conserved among several light-responsive promoters. One such sequence from a petunia Cab promoter is able to compete with as-2 for factor binding. In transgenic plants, a tetramer of as-2 is able to confer leaf expression when fused 5' to the -90 derivative of the 35S promoter. The expression is not dependent on light and, thus, the as-2 tetramer does not function as a light-responsive element in this context. Histochemical localization of the reporter gene product suggests that the as-2 tetramer directs expression in trichomes, vascular elements, and epidermal and mesophyll cells.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-16578811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-17770331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-17835349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-2535461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-2651885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-2813365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-2822486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-2843294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-3226423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-3697087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-3862099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2535536-7139714
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1040-4651
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1147-56
|
| pubmed:dateRevised |
2010-9-10
|
| pubmed:meshHeading |
pubmed-meshheading:2535536-Base Sequence,
pubmed-meshheading:2535536-Brassica,
pubmed-meshheading:2535536-DNA, Viral,
pubmed-meshheading:2535536-DNA-Binding Proteins,
pubmed-meshheading:2535536-Gene Expression Regulation,
pubmed-meshheading:2535536-Molecular Sequence Data,
pubmed-meshheading:2535536-Mosaic Viruses,
pubmed-meshheading:2535536-Promoter Regions, Genetic,
pubmed-meshheading:2535536-Sequence Homology, Nucleic Acid
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
ASF-2: a factor that binds to the cauliflower mosaic virus 35S promoter and a conserved GATA motif in Cab promoters.
|
| pubmed:affiliation |
Laboratory of Plant Molecular Biology, Rockefeller University, New York 10021-6399.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|