Linked Life Data

2535493

Source:http://linkedlifedata.com/resource/pubmed/id/2535493

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-10-9
pubmed:abstractText
125I-Wheat germ agglutinin (WGA) binding parameters of human urothelial cell lines of different grades of transformation (TGrII and TGrIII) were compared. The values of association constant (Ka) and the number of binding sites/cell for HCV29 (TGrII) cell line were about 3 x 10(6) M-1 and over 4 x 10(7), respectively. Two TGrIII cell lines, HCV29T and Hu549 revealed lower values for Ka, and considerably higher numbers of binding sites/cell (about 3 x 10(8) and 2 x 10(8), respectively). Binding of 125I-WGA to total cellular proteins resolved by SDS-PAGE and transferred to nitrocellulose showed multiple diffused bands in the range of 58-180 kDa. Some of these bands were characteristic for TGrII cells (124 kDa) or TGrIII cells (135 and 148 kDa).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0282-0080
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
355-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Wheat germ agglutinin binding sites on human urothelial cells of different grades of transformation.
pubmed:affiliation
Department of Tumor Immunology, Polish Academy of Sciences, Wroclaw.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't