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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5-6
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pubmed:dateCreated |
1990-3-15
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pubmed:abstractText |
Conidia of Neurospora crassa are sensitive to the herbicide bialaphos at concentrations of 160 mg/l in Westergaard's or Fries' minimal media. Plasmid pJA4 was constructed by inserting a truncated bar gene from Streptomyces hygroscopicus fused to the his-3 promoter from N. crassa into pUC19. The bar gene in plasmid pJA4 confers resistance to bialaphos when transformants are selected on a medium containing bialaphos. The bar gene can be used as an additional dominant selectable marker for transformation of fungi.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0172-8083
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
369-72
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2532965-Anti-Bacterial Agents,
pubmed-meshheading:2532965-Blotting, Southern,
pubmed-meshheading:2532965-Drug Resistance, Microbial,
pubmed-meshheading:2532965-Genes, Bacterial,
pubmed-meshheading:2532965-Genes, Fungal,
pubmed-meshheading:2532965-Genetic Markers,
pubmed-meshheading:2532965-Neurospora,
pubmed-meshheading:2532965-Neurospora crassa,
pubmed-meshheading:2532965-Organophosphorus Compounds,
pubmed-meshheading:2532965-Plasmids,
pubmed-meshheading:2532965-Promoter Regions, Genetic,
pubmed-meshheading:2532965-Restriction Mapping,
pubmed-meshheading:2532965-Streptomyces,
pubmed-meshheading:2532965-Transformation, Genetic
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pubmed:year |
1989
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pubmed:articleTitle |
Bialaphos resistance as a dominant selectable marker in Neurospora crassa.
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pubmed:affiliation |
Department of Genetics, University of Georgia, Athens 30602.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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