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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4935
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pubmed:dateCreated |
1990-1-11
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pubmed:abstractText |
A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
246
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1275-81
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:2531466-Amino Acid Sequence,
pubmed-meshheading:2531466-Animals,
pubmed-meshheading:2531466-Antibodies, Monoclonal,
pubmed-meshheading:2531466-Antibody Specificity,
pubmed-meshheading:2531466-Antigen-Antibody Reactions,
pubmed-meshheading:2531466-Bacteriophage lambda,
pubmed-meshheading:2531466-Base Sequence,
pubmed-meshheading:2531466-Cloning, Molecular,
pubmed-meshheading:2531466-Escherichia coli,
pubmed-meshheading:2531466-Gene Amplification,
pubmed-meshheading:2531466-Gene Library,
pubmed-meshheading:2531466-Genetic Vectors,
pubmed-meshheading:2531466-Hemocyanin,
pubmed-meshheading:2531466-Immunoglobulin Fab Fragments,
pubmed-meshheading:2531466-Immunoglobulin Fragments,
pubmed-meshheading:2531466-Mice,
pubmed-meshheading:2531466-Molecular Sequence Data,
pubmed-meshheading:2531466-Organophosphorus Compounds,
pubmed-meshheading:2531466-Recombinant Proteins
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pubmed:year |
1989
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pubmed:articleTitle |
Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.
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pubmed:affiliation |
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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