Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6248
|
| pubmed:dateCreated |
1990-1-4
|
| pubmed:abstractText |
Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. The partial purification and characterization of HGF have been reported. We have demonstrated that pure HGF from rat platelets is a new growth factor effective at concentrations as low as 1 ng ml-1. The effects of HGF and epidermal growth factor (EGF) are additive. The activity of HGF is not species-specific, although it does not stimulate growth in Swiss 3T3 fibroblasts. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. Here we report the amino-acid sequence of human HGF determined by complementary DNA cloning and the expression of biologically active human HGF from COS-1 cells transfected with cloned cDNA. The nucleotide sequence of the human HGF cDNA reveals that both alpha- and beta-chains are contained in a single open reading frame coding for a pre-pro precursor protein of 728 amino acids.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0028-0836
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
23
|
| pubmed:volume |
342
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
440-3
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2531289-Amino Acid Sequence,
pubmed-meshheading:2531289-Animals,
pubmed-meshheading:2531289-Base Sequence,
pubmed-meshheading:2531289-Cell Division,
pubmed-meshheading:2531289-Cell Line,
pubmed-meshheading:2531289-Cells, Cultured,
pubmed-meshheading:2531289-Cloning, Molecular,
pubmed-meshheading:2531289-Gene Expression,
pubmed-meshheading:2531289-Genes,
pubmed-meshheading:2531289-Genetic Vectors,
pubmed-meshheading:2531289-Growth Substances,
pubmed-meshheading:2531289-Hepatocyte Growth Factor,
pubmed-meshheading:2531289-Humans,
pubmed-meshheading:2531289-Liver,
pubmed-meshheading:2531289-Mice,
pubmed-meshheading:2531289-Models, Genetic,
pubmed-meshheading:2531289-Molecular Sequence Data,
pubmed-meshheading:2531289-Monocytes,
pubmed-meshheading:2531289-Protein Processing, Post-Translational,
pubmed-meshheading:2531289-Rats,
pubmed-meshheading:2531289-Restriction Mapping,
pubmed-meshheading:2531289-Transfection
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Molecular cloning and expression of human hepatocyte growth factor.
|
| pubmed:affiliation |
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|