| pubmed-article:2531215 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2531215 | lifeskim:mentions | umls-concept:C0027740 | lld:lifeskim |
| pubmed-article:2531215 | lifeskim:mentions | umls-concept:C0596632 | lld:lifeskim |
| pubmed-article:2531215 | lifeskim:mentions | umls-concept:C0031715 | lld:lifeskim |
| pubmed-article:2531215 | lifeskim:mentions | umls-concept:C0034826 | lld:lifeskim |
| pubmed-article:2531215 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:2531215 | lifeskim:mentions | umls-concept:C1414967 | lld:lifeskim |
| pubmed-article:2531215 | lifeskim:mentions | umls-concept:C1514758 | lld:lifeskim |
| pubmed-article:2531215 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:2531215 | pubmed:dateCreated | 1990-1-3 | lld:pubmed |
| pubmed-article:2531215 | pubmed:abstractText | A characteristic feature of neurite formation is high expression of the phosphoprotein B-50/GAP43. Previous studies with growth cone membranes have indicated that this neuron-specific protein kinase C substrate may be involved in transmembrane signal transduction at the growth cone. We monitored the degree of phosphorylation of B-50 by quantitative B-50 immunoprecipitation from intact nerve growth cones, isolated from 5-day-old rat brain and prelabeled with 32P-orthophosphate. B-50 phosphorylation in nerve growth cones is stimulated by 4 beta-phorbol 12,13-dibutyrate (PDB) and 1,2-dioctanoylglycerol (DOG) in a concentration-dependent manner, but not by 4 alpha-phorbol 12,13-didecanoate (4 alpha-PDD). These results confirm that B-50 is a substrate of PKC in intact growth cones. Depolarization induced by 30 mM K+ produces a transient increase in B-50 phosphorylation, which is maximal after 15 sec and declines to basal level within 5 min. This rise in B-50 phosphorylation can be partially blocked by atropine (10(-3)-10(-4) M), suggesting the involvement of muscarinic receptors. Indeed, the cholinergic receptor agonist carbachol enhances B-50 phosphorylation in a concentration-dependent manner (50% at 10(-3) M). Since the effect of carbachol (10(-3) M) can be blocked by atropine (10(-7) M), we conclude that this increase in B-50 phosphorylation is mediated through activation of the muscarinic receptors on the growth cones. The carbachol-induced stimulation is further increased by concurrent K+-depolarization. The effects of carbachol and depolarization are additive. To our knowledge, this is the first report showing receptor-mediated effects on the PKC substrate B-50 in growth cones. Our data support the hypothesis that phosphorylation of B-50 by PKC is involved in signal transduction in nerve growth cones. | lld:pubmed |
| pubmed-article:2531215 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2531215 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2531215 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2531215 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2531215 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2531215 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2531215 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2531215 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2531215 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:2531215 | pubmed:issn | 0270-6474 | lld:pubmed |
| pubmed-article:2531215 | pubmed:author | pubmed-author:GispenW HWH | lld:pubmed |
| pubmed-article:2531215 | pubmed:author | pubmed-author:OestreicherA... | lld:pubmed |
| pubmed-article:2531215 | pubmed:author | pubmed-author:De GraanP NPN | lld:pubmed |
| pubmed-article:2531215 | pubmed:author | pubmed-author:Van HooffC... | lld:pubmed |
| pubmed-article:2531215 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2531215 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:2531215 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2531215 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2531215 | pubmed:pagination | 3753-9 | lld:pubmed |
| pubmed-article:2531215 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:2531215 | pubmed:meshHeading | pubmed-meshheading:2531215-... | lld:pubmed |
| pubmed-article:2531215 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2531215 | pubmed:articleTitle | Muscarinic receptor activation stimulates B-50/GAP43 phosphorylation in isolated nerve growth cones. | lld:pubmed |
| pubmed-article:2531215 | pubmed:affiliation | Division of Molecular Neurobiology, Rudolf Magnus Institute, University of Utrecht, The Netherlands. | lld:pubmed |
| pubmed-article:2531215 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2531215 | pubmed:publicationType | In Vitro | lld:pubmed |
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