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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1990-1-3
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pubmed:abstractText |
A characteristic feature of neurite formation is high expression of the phosphoprotein B-50/GAP43. Previous studies with growth cone membranes have indicated that this neuron-specific protein kinase C substrate may be involved in transmembrane signal transduction at the growth cone. We monitored the degree of phosphorylation of B-50 by quantitative B-50 immunoprecipitation from intact nerve growth cones, isolated from 5-day-old rat brain and prelabeled with 32P-orthophosphate. B-50 phosphorylation in nerve growth cones is stimulated by 4 beta-phorbol 12,13-dibutyrate (PDB) and 1,2-dioctanoylglycerol (DOG) in a concentration-dependent manner, but not by 4 alpha-phorbol 12,13-didecanoate (4 alpha-PDD). These results confirm that B-50 is a substrate of PKC in intact growth cones. Depolarization induced by 30 mM K+ produces a transient increase in B-50 phosphorylation, which is maximal after 15 sec and declines to basal level within 5 min. This rise in B-50 phosphorylation can be partially blocked by atropine (10(-3)-10(-4) M), suggesting the involvement of muscarinic receptors. Indeed, the cholinergic receptor agonist carbachol enhances B-50 phosphorylation in a concentration-dependent manner (50% at 10(-3) M). Since the effect of carbachol (10(-3) M) can be blocked by atropine (10(-7) M), we conclude that this increase in B-50 phosphorylation is mediated through activation of the muscarinic receptors on the growth cones. The carbachol-induced stimulation is further increased by concurrent K+-depolarization. The effects of carbachol and depolarization are additive. To our knowledge, this is the first report showing receptor-mediated effects on the PKC substrate B-50 in growth cones. Our data support the hypothesis that phosphorylation of B-50 by PKC is involved in signal transduction in nerve growth cones.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Atropine,
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/GAP-43 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0270-6474
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3753-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2531215-Animals,
pubmed-meshheading:2531215-Atropine,
pubmed-meshheading:2531215-Brain,
pubmed-meshheading:2531215-Carbachol,
pubmed-meshheading:2531215-GAP-43 Protein,
pubmed-meshheading:2531215-Kinetics,
pubmed-meshheading:2531215-Membrane Proteins,
pubmed-meshheading:2531215-Nerve Tissue Proteins,
pubmed-meshheading:2531215-Phorbol Esters,
pubmed-meshheading:2531215-Phosphoproteins,
pubmed-meshheading:2531215-Phosphorylation,
pubmed-meshheading:2531215-Potassium,
pubmed-meshheading:2531215-Rats,
pubmed-meshheading:2531215-Rats, Inbred Strains,
pubmed-meshheading:2531215-Receptors, Muscarinic,
pubmed-meshheading:2531215-Synaptic Membranes,
pubmed-meshheading:2531215-Synaptosomes
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pubmed:year |
1989
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pubmed:articleTitle |
Muscarinic receptor activation stimulates B-50/GAP43 phosphorylation in isolated nerve growth cones.
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pubmed:affiliation |
Division of Molecular Neurobiology, Rudolf Magnus Institute, University of Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article,
In Vitro
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