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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1989-12-8
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pubmed:databankReference |
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pubmed:abstractText |
A murine interferon gamma (IFN-gamma) receptor cDNA was isolated by screening a murine T-cell hybridoma library prepared in lambda gt10 with probes prepared from a human IFN-gamma receptor cDNA. The 2.1-kilobase (kb) cDNA encoded a serine-rich polypeptide of 477 amino acids that was 52% identical to the human protein. Southern and Northern (RNA) blot analyses indicated the presence of a single receptor gene and a single predominant 2.3-kb receptor transcript. Human embryonic kidney fibroblasts, stably transfected with the murine IFN-gamma receptor cDNA, expressed murine IFN-gamma receptors as detected by flow cytometry with either ligand or a receptor-specific monoclonal antibody. Nontransfected cells bound neither ligand nor antibody. Radioligand-binding analysis demonstrated that the transfectants expressed 530,000 murine IFN-gamma receptors per cell and bound murine IFN-gamma with a Ka of 1 x 10(9) M-1. However, despite high-level expression of murine IFN-gamma receptors, the transfected human cells responded only to human and not to murine IFN-gamma as detected by enhancement of major histocompatibility class I antigen expression and induction of antiviral activity. These results thus document the isolation and expression of a full-length murine IFN-gamma receptor cDNA and suggest that additional species-specific components may be necessary to form a biologically active IFN-gamma receptor.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-182074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2435837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2450953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2453573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2455298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2475121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2526123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2578513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2849552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2934408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2934482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2944578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2954164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2954953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2957306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2957307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2970637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-2971451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-3030393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-3107127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-3110281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-4356326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-6154869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-6198242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-6306768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-6330272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-6816896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-6941282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-719759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-728996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2530582-987581
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8497-501
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2530582-Amino Acid Sequence,
pubmed-meshheading:2530582-Animals,
pubmed-meshheading:2530582-Base Sequence,
pubmed-meshheading:2530582-Blotting, Northern,
pubmed-meshheading:2530582-Blotting, Southern,
pubmed-meshheading:2530582-Cloning, Molecular,
pubmed-meshheading:2530582-DNA,
pubmed-meshheading:2530582-Female,
pubmed-meshheading:2530582-Gene Expression,
pubmed-meshheading:2530582-Genes,
pubmed-meshheading:2530582-Humans,
pubmed-meshheading:2530582-Interferon-gamma,
pubmed-meshheading:2530582-Kinetics,
pubmed-meshheading:2530582-Mice,
pubmed-meshheading:2530582-Molecular Sequence Data,
pubmed-meshheading:2530582-Placenta,
pubmed-meshheading:2530582-Pregnancy,
pubmed-meshheading:2530582-Receptors, Immunologic,
pubmed-meshheading:2530582-Receptors, Interferon,
pubmed-meshheading:2530582-Recombinant Proteins,
pubmed-meshheading:2530582-Transfection
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pubmed:year |
1989
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pubmed:articleTitle |
Cloning and expression of the cDNA for the murine interferon gamma receptor.
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pubmed:affiliation |
Genentech, Inc., South San Francisco, CA 94080.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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