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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1989-12-21
|
| pubmed:abstractText |
Previous studies (Overton et al., Mutation Res., 1989) on specific revertibility of 81 his-3 mutants have shown a correlation between complementation pattern and presumed genetic alteration similar to that shown by ad-3B mutants. In the present study, restriction enzyme analyses were used to further characterize the genetic alterations in individual his-3 mutants. The restriction fragment banding patterns of the majority of mutants were identical with that shown by wild-type 74-OR23-1A and were consistent with expectations based on previous data suggesting that they resulted from single base-pair alterations (Overton et al., Mutation Res., 1989). His-3 mutants with altered banding patterns were only found among those with polarized complementation patterns or noncomplementing mutants. One of the mutants with a polarized complementation pattern, 1-189-83, and another noncomplementing mutant, 1-189-85, are associated with genetic alterations proximal to the his-3 locus. In one other mutant, 1-226-565 (with a polarized complementation pattern), an insertion of approx. 2 kb has occurred in the proximal region of the his-3 locus. Two other mutants, 1-155-270 and 1-155-276 (both noncomplementing), contained a large insertion of approx. 12.8 kb in the proximal region of the his-3 locus.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0027-5107
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
215
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
39-47
|
| pubmed:dateRevised |
2008-8-19
|
| pubmed:meshHeading |
pubmed-meshheading:2530448-Blotting, Southern,
pubmed-meshheading:2530448-DNA, Fungal,
pubmed-meshheading:2530448-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:2530448-Genes, Fungal,
pubmed-meshheading:2530448-Genetic Complementation Test,
pubmed-meshheading:2530448-Histidine,
pubmed-meshheading:2530448-Mutation,
pubmed-meshheading:2530448-Neurospora,
pubmed-meshheading:2530448-Neurospora crassa,
pubmed-meshheading:2530448-Nucleic Acid Hybridization,
pubmed-meshheading:2530448-Plasmids,
pubmed-meshheading:2530448-Restriction Mapping
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Classical and molecular genetic analyses of his-3 mutants of Neurospora crassa. II. Southern blot analyses and molecular mechanisms of mutagenicity.
|
| pubmed:affiliation |
Center for Life Sciences and Toxicology, Research Triangle Park, NC 27709-2194.
|
| pubmed:publicationType |
Journal Article
|