Linked Life Data

2530275

Source:http://linkedlifedata.com/resource/pubmed/id/2530275

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1989-12-15
pubmed:abstractText
The effects of the newly isolated bovine milk growth factor (MGF) which shows N-terminal homology to transforming growth factor beta 2 were compared with the effects of porcine transforming growth factor beta 1 and beta 2 (pTGF-beta 1 and -beta 2) on human T lymphocyte activation. Freshly isolated human PBMC were stimulated with either PHA, anti-CD3 + phorbol-12,13-dibutyrate (PDBu), or with a combination of ionomycin + PDBu. MGF, pTGF-beta 1, and pTGF-beta 2 decreased mitogen-induced [3H]thymidine incorporation by 30 to 75% in a dose-dependent manner. The maximum degree of inhibition was obtained at 1 ng/ml (40 pM) and could not be increased by increasing the concentration of teh transforming growth factor 10-fold. Stimulation of fresh T cells with the recall Ag tetanus toxoid was also inhibited (85%) by MGF at pM concentrations as was the proliferation of a human T cell clone specific for purified protein derivative. The effects of MGF and pTGF-beta 1 on anti-CD3-mediated increase of intracellular Ca2+ (Cai2+) was investigated by using the Fura-2 method. Neither MGF nor pTGF-beta 1 inhibited this increase in Cai2+ induced by a mitogenic concentration of anti-CD3 antibody. In order to determine whether TGF-beta preferentially inhibited the CD4+ or CD8+ subpopulation of human T cells, a limiting dilution analysis system, which allows every T cell to proliferate, was used. pTGF-beta 1 at a concentration of 5 ng/ml decreased the frequency of proliferating T cell precursors of both the CD4+ and CD8+ subsets to a similar extent. Furthermore, MGF, pTGF-beta 1, and pTGF-beta 2 also decreased IL-2 mediated [3H]thymidine incorporation into human PBL Con A blasts and the IL-4-mediated [3H]thymidine incorporation of purified T lymphocytes costimulated with PDBu by 70%. In conclusion, bovine MGF exerts suppressive effects on human T cells stimulated with Ag, mitogens, or interleukins, and the degree of T cell suppression is similar (or identical) to those of pTGF-beta 1 or -beta 2.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3258-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:2530275-Animals, pubmed-meshheading:2530275-Antigens, CD3, pubmed-meshheading:2530275-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:2530275-Calcium, pubmed-meshheading:2530275-Cattle, pubmed-meshheading:2530275-Growth Substances, pubmed-meshheading:2530275-Humans, pubmed-meshheading:2530275-Interleukin-2, pubmed-meshheading:2530275-Interleukin-4, pubmed-meshheading:2530275-Leukocyte Count, pubmed-meshheading:2530275-Lymphocyte Activation, pubmed-meshheading:2530275-Lymphocytes, pubmed-meshheading:2530275-Milk Proteins, pubmed-meshheading:2530275-Phenotype, pubmed-meshheading:2530275-Receptors, Antigen, T-Cell, pubmed-meshheading:2530275-Stem Cells, pubmed-meshheading:2530275-Suppressor Factors, Immunologic, pubmed-meshheading:2530275-Swine, pubmed-meshheading:2530275-T-Lymphocytes, pubmed-meshheading:2530275-Thymidine, pubmed-meshheading:2530275-Transforming Growth Factors
pubmed:year
1989
pubmed:articleTitle
Comparison of the immunosuppressive properties of milk growth factor and transforming growth factors beta 1 and beta 2.
pubmed:affiliation
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't