Linked Life Data

2529135

Source:http://linkedlifedata.com/resource/pubmed/id/2529135

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-10-30
pubmed:abstractText
Two Theileria parva-specific bovine helper T cell clones were used to identify T. parva-derived antigens expressed on the surface of schizont infected lymphoblastoid cells. Although the clones proliferated in response to both the immunizing (Muguga) and heterologous stocks of T. parva, the patterns of the responses differed, showing that the two clones recognized different antigenic epitopes. Both clones were stimulated by autologous infected cells, without an additional source of antigen-presenting cells, as well as by purified schizonts and by a subcellular membrane fraction prepared from infected lymphoblastoid cells, when antigen-presenting cells were present. The membrane fraction was shown to be enriched for schizont membranes as indicated by the presence of a schizont surface antigen detected by immunoblotting using a schizont-specific monoclonal antibody. Elimination of schizonts with the anti-theilerial drug, parvaquone, resulted in reduced antigenicity of the membrane fraction as detected by both the T cell clones and the schizont-specific monoclonal antibody. We conclude that the T. parva-infected cell surface antigens recognized by the T cell clones are of schizont membrane origin. Although the antigens have not yet been characterized biochemically, the monoclonal antibody-specific epitope appears to be distinguishable from the T cell epitopes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-4894
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
234-48
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Theileria parva: bovine helper T cell clones specific for both infected lymphocytes and schizont membrane antigens.
pubmed:affiliation
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
pubmed:publicationType
Journal Article