Linked Life Data

2528991

Source:http://linkedlifedata.com/resource/pubmed/id/2528991

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1989-11-15
pubmed:abstractText
ATP-synthesis catalyzed by proteoliposomes incorporating Rhodospirillum rubrum F0F1 was driven by artificially applied electrochemical proton gradients. The time-course of ATP-synthesis was followed continuously by means of firefly luciferase. Correction methods were developed which allow one to calculate the initial rate of ATP-synthesis from the observed luminescence kinetics. The following results were obtained: (1) ATP-synthesis occurred above a threshold delta mu H+ of 90 mV; this threshold is not imposed by the activation requirement of the enzyme; (2) delta psi and delta pH appear to be equivalent as driving forces for ATP-synthesis if allowance is made for the effect of the electrical capacitance of the liposome membrane on the distribution of K+ at equilibrium; and (3) the highest rate observed so far is 200 mol ATP per mol F0F1 per s.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
976
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
150-60
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
ATP-synthesis by proteoliposomes incorporating Rhodospirillum rubrum F0F1 as measured with firefly luciferase: dependence on delta psi and delta pH.
pubmed:affiliation
Vrije Universiteit Brussel, Faculty of Sciences, Laboratory of Biophysics, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't