Linked Life Data

2528589

Source:http://linkedlifedata.com/resource/pubmed/id/2528589

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1989-10-20
pubmed:abstractText
The murine B cell FcR for IgG (Fc gamma RII) is a membrane glycoprotein reported to mediate inhibition of B cell activation and differentiation. We show that IL-4 inhibits the enhanced expression of Fc gamma RII by LPS-stimulated B cells. This activity is completely reversed by anti-IL-4 mAb and is specific, in that multiple other lymphokines tested do not exert a similar effect. This effect of IL-4 is apparent by day 1 of culture, although maximal inhibition occurs on day 4 at a concentration of 500 U/ml. The IL-4-induced inhibition of enhanced Fc gamma RII expression by LPS stimulation observed on day 4 of culture is associated with a significant reduction in the steady state level of Fc gamma RII beta gene-specific mRNA. IFN-gamma which inhibits many of the effects of IL-4 on B cells, does not reverse the IL-4-induced inhibition of Fc gamma RII membrane expression nor the levels of beta gene-specific mRNA. Fc gamma RII expression is significantly increased in B cells stimulated with antigen-specific, CD4+ T cell clones of the Th1 type (i.e., IL-2 and IFN-gamma-producing). By contrast, three different Th2 clones (i.e., IL-4-producing) fail to stimulate an increase in Fc gamma RII levels. Anti-IL-4 mAb significantly enhanced Fc gamma RII expression by Th2-stimulated B cells indicating that IL-4 was the active, inhibitory, substance produced by the Th2 cells. Supernatants from stimulated Th2 clones inhibited the enhanced expression of Fc gamma RII by LPS-stimulated B cells and this activity was completely reversed by anti-IL-4 mAb. By contrast, supernatants from stimulated Th1 clones further enhanced Fc gamma RII expression by LPS-stimulated B cells. The differential regulation of B cell Fc gamma RII expression by Th subsets may play an important role in the regulation of humoral immunity by altering the sensitivity of B cells to IgG immune complex-mediated inhibition of B cell activation and differentiation in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2133-41
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2528589-Animals, pubmed-meshheading:2528589-Antigens, Differentiation, pubmed-meshheading:2528589-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:2528589-B-Lymphocytes, pubmed-meshheading:2528589-Binding, Competitive, pubmed-meshheading:2528589-Cell Line, pubmed-meshheading:2528589-Female, pubmed-meshheading:2528589-Genes, Immunoglobulin, pubmed-meshheading:2528589-Immunoglobulin G, pubmed-meshheading:2528589-Interferon-gamma, pubmed-meshheading:2528589-Interleukin-4, pubmed-meshheading:2528589-Interleukins, pubmed-meshheading:2528589-Kinetics, pubmed-meshheading:2528589-Lipopolysaccharides, pubmed-meshheading:2528589-Lymphocyte Activation, pubmed-meshheading:2528589-Membrane Proteins, pubmed-meshheading:2528589-Mice, pubmed-meshheading:2528589-Mice, Inbred C3H, pubmed-meshheading:2528589-Mice, Inbred DBA, pubmed-meshheading:2528589-Phenotype, pubmed-meshheading:2528589-RNA, Messenger, pubmed-meshheading:2528589-Receptors, Fc, pubmed-meshheading:2528589-Receptors, IgG, pubmed-meshheading:2528589-T-Lymphocytes, Helper-Inducer
pubmed:year
1989
pubmed:articleTitle
Differential regulation of murine B cell Fc gamma RII expression by CD4+ T helper subsets.
pubmed:affiliation
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.