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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-9-22
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pubmed:abstractText |
Two populations of alkaline-borohydride-reduced keratan sulphate (KS) chains were prepared from the two peptido-keratan sulphate trypsin fragments of proteoglycan aggregates isolated from bovine femoral head cartilage (6-year-old animals). Each population was separated by high-performance ion-exchange chromatography on a Pharmacia Mono-Q column into eight pools, Q1-Q8. These were analysed by gel permeation chromatography, radioimmunoassay with the monoclonal antibody MZ15, and 500 MHz 1H n.m.r. spectroscopy. Upon chromatography on Sephadex G-75 the Mono-Q fractions were shown to increase in hydrodynamic size progressively from Q1 to Q8 for both KS populations. For each population the strongest antigenic response with the anti-KS monoclonal antibody MZ15 was expressed by the two fractions of greatest size and charge density, Q7 and Q8. Proton n.m.r. spectroscopic studies on the two series of fractions demonstrated: (i) a progressive increase in the level of galactose sulphation from Q1 to Q8, (ii) the presence of approximately one alpha(1-3)-linked fucose residue per chain in every sample, and (iii) the presence of N-acetylneuraminic acids in three discrete environments, two alpha(2-3)- and one alpha(2-6)-linked in every sample. The results are discussed in terms of a possible heterogeneity in the carbohydrate-protein linkage region of keratan sulphates from bovine articular cartilage.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-139406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-13971270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-14284693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2423332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2430799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2447873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-276862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2935158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2940089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2947572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2952451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2963618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2965877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-2966847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-3038077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-3159736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-3160390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-3435493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-3693370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-4226136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-4226476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-4230492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-4261398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-4281652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-518094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-5637714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-6215207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-6223038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528344-7391006
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
260
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
277-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2528344-Animals,
pubmed-meshheading:2528344-Antibodies, Monoclonal,
pubmed-meshheading:2528344-Cartilage, Articular,
pubmed-meshheading:2528344-Cattle,
pubmed-meshheading:2528344-Chromatography, Gel,
pubmed-meshheading:2528344-Chromatography, Ion Exchange,
pubmed-meshheading:2528344-Glycosaminoglycans,
pubmed-meshheading:2528344-Keratan Sulfate,
pubmed-meshheading:2528344-Magnetic Resonance Spectroscopy
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pubmed:year |
1989
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pubmed:articleTitle |
Structural and immunological studies of keratan sulphates from mature bovine articular cartilage.
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pubmed:affiliation |
Department of Biological Sciences, University of Lancaster, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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