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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1989-10-12
|
| pubmed:abstractText |
D1 dopamine receptors were identified in membranes of human nucleus caudatus, nucleus accumbens, amygdala, and globus pallidus, by the specific binding of [3H](+)-R-8-chloro-2,3,4,5-tetrahydro-3-methyl-5-phenyl-1H-benzazepine-7 -ol [( 3H]SCH 23390). In these four brain regions, dopamine/[3H]SCH 23390 competition binding curves were computer-analyzed to a two-site model, distinguishing a high- (RH) and low- (RL) affinity site for dopamine. The ability of guanine nucleotides (0.4 mM GTP or 0.1 mM 5'-guanylylimidodiphosphate) to provoke a conversion of RH into RL was different between these brain regions. In amygdala, a complete conversion was seen, whereas there was no guanine nucleotide-effect on RH in globus pallidus. In nucleus caudatus and nucleus accumbens, guanine nucleotides provoked only a partial conversion of RH into RL, suggesting that these brain regions may contain guanine nucleotide-sensitive and -insensitive receptors. Heating of the membranes at 60 degrees C for 5 min had the same effect as guanine nucleotides. The pharmacological profiles of the guanine nucleotide-sensitive and -insensitive D1 receptors were similar, suggesting that D1 receptors in human brain are heterogeneous only with respect to their effector-coupling mechanism: guanine nucleotide-sensitive receptors, which are capable of undergoing functional coupling with Gs, and guanine nucleotide-insensitive receptors, which are not.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzazepines,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D1,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
53
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1096-102
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2527968-Aged,
pubmed-meshheading:2527968-Aged, 80 and over,
pubmed-meshheading:2527968-Amygdala,
pubmed-meshheading:2527968-Autoradiography,
pubmed-meshheading:2527968-Benzazepines,
pubmed-meshheading:2527968-Brain,
pubmed-meshheading:2527968-Caudate Nucleus,
pubmed-meshheading:2527968-Cerebral Infarction,
pubmed-meshheading:2527968-Dopamine,
pubmed-meshheading:2527968-Dopamine Antagonists,
pubmed-meshheading:2527968-Female,
pubmed-meshheading:2527968-Globus Pallidus,
pubmed-meshheading:2527968-Guanosine Triphosphate,
pubmed-meshheading:2527968-Hot Temperature,
pubmed-meshheading:2527968-Humans,
pubmed-meshheading:2527968-Kinetics,
pubmed-meshheading:2527968-Male,
pubmed-meshheading:2527968-Middle Aged,
pubmed-meshheading:2527968-Nucleus Accumbens,
pubmed-meshheading:2527968-Organ Specificity,
pubmed-meshheading:2527968-Receptors, Dopamine,
pubmed-meshheading:2527968-Receptors, Dopamine D1,
pubmed-meshheading:2527968-Reference Values,
pubmed-meshheading:2527968-Tritium
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
In human brain two subtypes of D1 dopamine receptors can be distinguished on the basis of differences in guanine nucleotide effect on agonist binding.
|
| pubmed:affiliation |
Department of Neurology, Akademisch Ziekenhuis, Vrije Universiteit Brussel, Belgium.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|