Linked Life Data

2527086

Source:http://linkedlifedata.com/resource/pubmed/id/2527086

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-9-15
pubmed:abstractText
A new human cell line, WSU-BL, was established from a malignant ascitic fluid occurring in a patient with Burkitt's lymphoma. The established line grows in a single-cell suspension with a doubling time of 19 hours and expresses L3 morphologic features by the French-American-British classification. Immunologic study revealed that WSU-BL cells express IgM-lambda both in the cytoplasm and on the surface and react with monoclonal antibodies to B-cell antigens (B1, B4, BL3, BL4, HLA-DR, and common acute lymphoblastic leukemia antigen [CALLA]). These cells are negative for T-cell and myeloid/monocyte antigens as well as Epstein-Barr virus nuclear antigen (EBNA). These results suggest that WSU-BL corresponds to an intermediate stage of B-cell differentiation. Both fresh tumor and WSU-BL cells had a hyperdiploid karyotype carrying the 8;14 chromosome translocation. Molecular studies showed that WSU-BL has a rearrangement of c-myc proto-oncogene and expresses c-myc RNA. Phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA) and interferon-gamma (IFN-gamma) were able to induce several phenotypic changes on WSU-BL cells. Two-dimensional gel electrophoresis of total cellular protein showed that either TPA or IFN-gamma induced both the synthesis or loss of several proteins. Analysis of the protein patterns indicated that some proteins were uniquely responsive to either TPA or IFN-gamma and others were common to both. This cell line should be valuable for future studies of cell proliferation, differentiation, and oncogenesis concerning this neoplasm.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-543X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1041-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2527086-Adult, pubmed-meshheading:2527086-Antigens, Differentiation, pubmed-meshheading:2527086-Antigens, Neoplasm, pubmed-meshheading:2527086-Antigens, Surface, pubmed-meshheading:2527086-Ascitic Fluid, pubmed-meshheading:2527086-B-Lymphocytes, pubmed-meshheading:2527086-Burkitt Lymphoma, pubmed-meshheading:2527086-Cell Differentiation, pubmed-meshheading:2527086-Cell Division, pubmed-meshheading:2527086-Cytoplasm, pubmed-meshheading:2527086-Humans, pubmed-meshheading:2527086-Immunoglobulin M, pubmed-meshheading:2527086-Immunoglobulin lambda-Chains, pubmed-meshheading:2527086-Interferon-gamma, pubmed-meshheading:2527086-Karyotyping, pubmed-meshheading:2527086-Male, pubmed-meshheading:2527086-Neprilysin, pubmed-meshheading:2527086-Proto-Oncogene Proteins, pubmed-meshheading:2527086-Proto-Oncogene Proteins c-myc, pubmed-meshheading:2527086-Tetradecanoylphorbol Acetate, pubmed-meshheading:2527086-Translocation, Genetic, pubmed-meshheading:2527086-Tumor Cells, Cultured
pubmed:year
1989
pubmed:articleTitle
Establishment and characterization of a new human Burkitt's lymphoma cell line (WSU-BL).
pubmed:affiliation
Department of Internal Medicine, Wayne State University School of Medicine, Detroit, MI.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Case Reports, Research Support, Non-U.S. Gov't