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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1989-8-30
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pubmed:databankReference |
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pubmed:abstractText |
The nucleotide sequence of a staphylococcal bacteriophage L54a DNA fragment containing genes involved in site-specific recombination was determined. Mutations generated by in vitro mutagenesis were used to map and characterize the int and xis genes. The site-specific recombination functions are tightly clustered within a 1.75-kilobase stretch of DNA fragment with the gene order of attP-int-xis. The int and xis genes are transcribed divergently. The Int protein deduced from the nucleotide sequence has a molecular weight of 41,000. Int is a basic protein with 354 amino acids of which 72 are basic and 38 are acidic. The Xis protein consists of only 59 amino acids with a molecular weight of 7,180. Unlike the Xis proteins of the lambdoid bacteriophages which are all basic proteins, L54a Xis is an acidic protein containing 13 acidic and 8 basic amino acids. The Int protein is required in both integrative and excisive reactions, whereas Xis is only required in excisive reaction. A well-conserved 40-residue region, including three perfectly conserved residues found in 15 site-specific recombinases of the integrase family that have been characterized, was also found in the L54a Int protein.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-107388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-1184573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-1186174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-2522092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-2836392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-2942938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-2958633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-2960665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-2995312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-3009394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-3011407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-3027659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-3474623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-3491212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-353481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-3839563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-4275766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-451050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-4598299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-6226876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-6232171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-6235151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-6253947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-6269464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-6446713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-6857997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526804-7220647
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4146-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2526804-Amino Acid Sequence,
pubmed-meshheading:2526804-Base Sequence,
pubmed-meshheading:2526804-DNA, Viral,
pubmed-meshheading:2526804-Escherichia coli,
pubmed-meshheading:2526804-Genes, Viral,
pubmed-meshheading:2526804-Genetic Complementation Test,
pubmed-meshheading:2526804-Molecular Sequence Data,
pubmed-meshheading:2526804-Mutation,
pubmed-meshheading:2526804-Plasmids,
pubmed-meshheading:2526804-Restriction Mapping,
pubmed-meshheading:2526804-Staphylococcus Phages,
pubmed-meshheading:2526804-Staphylococcus aureus,
pubmed-meshheading:2526804-Viral Proteins
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pubmed:year |
1989
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pubmed:articleTitle |
Nucleotide sequence and genetic characterization of staphylococcal bacteriophage L54a int and xis genes.
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pubmed:affiliation |
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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