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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1989-8-16
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pubmed:abstractText |
The cellular distributions of the kidney form of the erythrocyte band 3 chloride/bicarbonate exchanger and the kidney vacuolar H+-transporting ATPase were examined in rat kidney collecting duct by immunocytochemical staining of adjacent semithin sections. Polyclonal anti-peptide antibodies directed against two regions of murine erythroid band 3 gave a pattern of basolateral labeling similar to that seen with antibodies directed against the entire protein. In the medullary collecting duct almost all intercalated cells expressed basolateral membrane band 3 and displayed apical membrane H+-ATPase. In the cortical collecting duct and the connecting segment, band 3 labeling was restricted to a subpopulation of intercalcated cells. In the cortical collecting duct 46% of intercalated cells had apical H+-ATPase and basolateral band 3. Cells that had either basolateral or diffuse cytoplasmic staining for H+-ATPase were all band 3-negative and accounted for 53% of the intercalated cells. In addition, occasional intercalated cells with apical H+-ATPase appeared to lack basolateral band 3. These results demonstrate the coexpression of H+-ATPase and band 3 in opposite plasma membrane domains of a subpopulation of intercalated cells that are probably the acid-excreting (type A) cells. All other intercalated cells lacked immunoreactive band 3 and probably include the bicarbonate-excreting (type B) cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2410791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2415824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2462075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2466837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2542243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2582000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2722777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2889740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2890634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2893294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2904451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2933809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2961771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2996366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-2999293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-3039855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-3077542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-3182834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-3184173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-3290207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-3303972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-3326961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-3394806,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-3478687,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-6318566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-6408932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-6793594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-6812435,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2526338-7112126
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5429-33
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2526338-Animals,
pubmed-meshheading:2526338-Anion Exchange Protein 1, Erythrocyte,
pubmed-meshheading:2526338-Antibodies,
pubmed-meshheading:2526338-Antibody Specificity,
pubmed-meshheading:2526338-Blotting, Western,
pubmed-meshheading:2526338-Erythrocyte Membrane,
pubmed-meshheading:2526338-Fluorescent Antibody Technique,
pubmed-meshheading:2526338-Humans,
pubmed-meshheading:2526338-Kidney Cortex,
pubmed-meshheading:2526338-Kidney Tubules,
pubmed-meshheading:2526338-Kidney Tubules, Collecting,
pubmed-meshheading:2526338-Mice,
pubmed-meshheading:2526338-Proton-Translocating ATPases,
pubmed-meshheading:2526338-Rats,
pubmed-meshheading:2526338-Rats, Inbred Strains,
pubmed-meshheading:2526338-Vacuoles
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pubmed:year |
1989
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pubmed:articleTitle |
Subtypes of intercalated cells in rat kidney collecting duct defined by antibodies against erythroid band 3 and renal vacuolar H+-ATPase.
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pubmed:affiliation |
Whitehead Institute, Cambridge, MA 02142.
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pubmed:publicationType |
Journal Article
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