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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
|
pubmed:dateCreated |
1989-7-6
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pubmed:abstractText |
The IL-1R on murine T cells is an 80-kDa cell surface glycoprotein which binds both IL-1 alpha and IL-1 beta. We have recently isolated a cDNA clone encoding this molecule. From the primary sequence mature receptor is predicted to be a 557 residue integral membrane protein with a 319 residue carbohydrate-rich extracellular region. We have constructed a cDNA clone encoding this region of the protein (residues 1 to 316). Expression of this cDNA in HeLa cells leads to secretion of a soluble IL-1 alpha binding protein into the culture medium. Quantitative binding experiments with the truncated receptor show that it possesses IL-1 binding properties which are indistinguishable from those of full length IL-1R. Gel filtration chromatography experiments show that a complex can be formed between a single truncated receptor molecule and a single IL-1 alpha molecule.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
142
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4314-20
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2524527-Animals,
pubmed-meshheading:2524527-Chromatography, Gel,
pubmed-meshheading:2524527-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2524527-Extracellular Matrix,
pubmed-meshheading:2524527-Humans,
pubmed-meshheading:2524527-Interleukin-1,
pubmed-meshheading:2524527-Mice,
pubmed-meshheading:2524527-Molecular Weight,
pubmed-meshheading:2524527-Radioligand Assay,
pubmed-meshheading:2524527-Receptors, Immunologic,
pubmed-meshheading:2524527-Receptors, Interleukin-1,
pubmed-meshheading:2524527-Recombinant Proteins,
pubmed-meshheading:2524527-Solubility
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pubmed:year |
1989
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pubmed:articleTitle |
Retention of ligand binding activity by the extracellular domain of the IL-1 receptor.
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pubmed:affiliation |
Immunex Corporation, Seattle, WA 98101.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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