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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-4-24
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pubmed:abstractText |
We have studied the control and significance of IL-1 production in human leukocyte cultures during accessory cell-dependent, T lymphocyte mitogenesis using sensitive bioassays and immunolabeling techniques. In primary antigen-dependent systems like the MLR, IL-1 production was not detected in accessory cells (monocytes, dendritic cells) or T cells, suggesting that it is not an early product in these responses. However, monocytes could be induced to make IL-1 after interacting with sensitized antigen-specific T cells. Both alloreactive T cell clones or freshly prepared lymphoblasts induced IL-1 provided the monocytes carried the HLA-DR antigens to which the T cells were initially sensitized. Even in these circumstances, dendritic cells and B cells failed to make IL-1. The mechanism whereby activated T cells induce IL-1 in monocytes was explored. Supernatants from cocultures of monocytes and T cells or several recombinant cytokines induced little or no IL-1. A more potent antigen independent pathway of IL-1 induction was identified. IL-1 could be induced in third-party HLA-DR nonspecific monocytes in cocultures of alloreactive T cell clones or blasts and HLA-DR-specific dendritic cells. The induction was factor independent since dendritic cells and T blasts placed in a chamber separate from third-party monocytes by a semipermeable membrane did not induce monocyte IL-1. These results suggest that a cell contact mechanism rather than an IL-1-inducing factor leads to IL-1 production. The role of IL-1 in T cell proliferation was tested with a polyclonal anti-IL-1 antibody. The antibody failed to block the proliferation of primary T cells, or alloreactive T cell clones and blasts stimulated with HLA-specific monocytes or dendritic cells, even though IL-1 in the medium was neutralized.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-2431094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-2842397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-2946770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-2950198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-2953844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-2960773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-2962741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-2962742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-2969406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3114369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3127525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3128630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3257250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3260544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3263397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3486911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3494059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3494192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3495797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3499611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3517219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3520562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3926880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-3928742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2522495-6190974
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1121-36
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2522495-Antigen-Presenting Cells,
pubmed-meshheading:2522495-B-Lymphocytes,
pubmed-meshheading:2522495-Concanavalin A,
pubmed-meshheading:2522495-Dendritic Cells,
pubmed-meshheading:2522495-HLA-DR Antigens,
pubmed-meshheading:2522495-Humans,
pubmed-meshheading:2522495-Immunoassay,
pubmed-meshheading:2522495-Interleukin-1,
pubmed-meshheading:2522495-Kinetics,
pubmed-meshheading:2522495-Leukocytes,
pubmed-meshheading:2522495-Lipopolysaccharides,
pubmed-meshheading:2522495-Lymphocyte Activation,
pubmed-meshheading:2522495-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:2522495-Monocytes,
pubmed-meshheading:2522495-T-Lymphocytes
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pubmed:year |
1989
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pubmed:articleTitle |
Interleukin 1 production during accessory cell-dependent mitogenesis of T lymphocytes.
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pubmed:affiliation |
Rockefeller University, New York, New York 10021.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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