Linked Life Data

2521882

Source:http://linkedlifedata.com/resource/pubmed/id/2521882

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-3-28
pubmed:databankReference
pubmed:abstractText
Previously we have isolated about 60 novel cDNA clones whose corresponding mRNAs are induced by mitogenic activation in human peripheral blood T cells. Here we describe the primary structure and regulation of two such cloned genes, pAT 464 and pAT 744, which may encode new lymphokines/cytokines. Similar to IL-2, both genes require the synergy of agents such as PHA and PMA for optimal expression, and, in addition, the induction of both is sensitive to the immunosuppressive drug cyclosporin A. The two genes can be expressed in T cells, B cells, and the promyelocytic cell line HL60, but they are not expressed in human fibroblasts, suggesting that their expression is restricted to hematopoietic lineages. The predicted peptides encoded by these two clones feature hydrophobic N-terminal leaders characteristic of secreted proteins. The predicted size of both proteins is about 8 kDa upon cleavage of the putative leader peptide. pAT 464 and pAT 744 are very similar to each other and also share some critical amino acid similarity with a newly emerging family of secreted factors including connective tissue activating factor III, platelet factor 4, an IFN-gamma-induced factor, macrophage inflammatory protein, and a factor chemotactic to neutrophils (3-10C, monocyte-derived neutrophil chemotactic factor, neutrophil-activating factor). Some of these factors have been shown to display functions associated with an inflammatory response and/or have mitogenic activities. Collectively, the data presented here suggest that pAT 464 and pAT 744 encode novel lymphokines/cytokines which may play roles during an immune response similar to those enacted by these structurally related factors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1582-90
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:2521882-Amino Acid Sequence, pubmed-meshheading:2521882-Base Sequence, pubmed-meshheading:2521882-Biological Factors, pubmed-meshheading:2521882-Chemotactic Factors, pubmed-meshheading:2521882-Cloning, Molecular, pubmed-meshheading:2521882-DNA, pubmed-meshheading:2521882-Gene Expression Regulation, pubmed-meshheading:2521882-Genes, pubmed-meshheading:2521882-Humans, pubmed-meshheading:2521882-Interleukin-8, pubmed-meshheading:2521882-Lymphocyte Activation, pubmed-meshheading:2521882-Mitogens, pubmed-meshheading:2521882-Molecular Sequence Data, pubmed-meshheading:2521882-Peptides, pubmed-meshheading:2521882-Platelet Factor 4, pubmed-meshheading:2521882-Sequence Homology, Nucleic Acid, pubmed-meshheading:2521882-T-Lymphocytes, pubmed-meshheading:2521882-beta-Thromboglobulin
pubmed:year
1989
pubmed:articleTitle
Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors.
pubmed:affiliation
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't