Linked Life Data

2521798

Source:http://linkedlifedata.com/resource/pubmed/id/2521798

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-3-29
pubmed:abstractText
The microelectrophoretic mobility of corn root plasma membranes and the inhibition of the Mg+2-ATPase by vanadate were investigated under different ionic conditions. The Mg2+-ATPase was uncompetitively inhibited and a 10-fold variation of the apparent inhibition constant was observed, depending on the addition of K+ and Mg2+. The determination of the zeta potential indicated that a 5-fold decrease of the apparent inhibition constant was due to aspecific electrostatic interactions of the vanadate anion and the negative charge of the membrane. The screening and masking effects of 6 mM free Mg2+ totally abolished electrostatic interactions and allowed the direct determination of the intrinsic vanadate inhibition constant (KIi). On the other hand, a specific, non-electrostatic, effect of K+ caused a 2-fold decrease of the inhibition constant in addition to the electrostatic effect. Finally, the electrostatic analysis indicates that the Mg2+-ATPase is inhibited by the monomeric bivalent anion HVO4(2-).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
979
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
46-52
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Electrostatic analysis of effects of ions on the inhibition of corn root plasma membrane Mg2+-ATPase by the bivalent orthovanadate.
pubmed:affiliation
Biochimie et Physiologie Végétales, INRA (CNRS URA 198), ENSAM, Montpellier, France.
pubmed:publicationType
Journal Article