2521426

pubmed:Citation

1 Pt 1

The sustained aldosterone secretory response to angiotensin II (ANG II) depends on receptor-mediated increases in membrane diglyceride (DG) and an increase in calcium influx rate. These signals serve to activate membrane-associated protein kinase C (PKC) and result in enhanced phosphorylation of a unique set of proteins. These events can be mimicked by the addition of a phorbol ester, 12-O-tetra decanoyl phorbol 13-acetate (TPA), and a calcium ionophore, A23187, that bypass the initial receptor-associated events. We studied the inhibitory action of atrial natriuretic peptide (4-28 hANP) on the sustained secretory response to ANG II in isolated bovine adrenal glomerulosa cells. Although 10 nM ANP inhibited aldosterone secretion, it did not significantly alter the ANG II-elicited rise in 45Ca2+ influx rate [control (CON): 0.44 +/- 0.06; ANG II: 1.11 +/- 0.12 (P less than 0.001); ANG II + ANP: 1.18 +/- 0.14], the steady-state level of aequorin luminescence [intracellular [Ca2+] ([Ca2+]i)], or the rise in cellular DG content [CON: 0.132 +/- 0.01; ANG II: 0.194 +/- 0.01 (P less than 0.005); ANG II + ANP: 0.202 +/- 0.01 nmol/10(6) cells]. IN addition, ANP was able to inhibit aldosterone secretion stimulated by the combined addition of A23187 + TPA. When protein phosphorylation in the ANP-inhibited cells was evaluated, ANG II-induced protein phosphorylation events were preserved. In contrast to the effect of ANP, the calcium channel blocker nitrendipine abolished the ANG II-induced rise in 45Ca2+ influx rate, reduced the steady-state level of [Ca2+]i, and returned the phosphoproteins to their control states.(ABSTRACT TRUNCATED AT 250 WORDS)

eng

IM

MEDLINE

0002-9513

Print

NLM

C89-95

pubmed-meshheading:2521426-Aequorin, pubmed-meshheading:2521426-Aldosterone, pubmed-meshheading:2521426-Angiotensin II, pubmed-meshheading:2521426-Animals, pubmed-meshheading:2521426-Atrial Natriuretic Factor, pubmed-meshheading:2521426-Calcimycin, pubmed-meshheading:2521426-Calcium, pubmed-meshheading:2521426-Cattle, pubmed-meshheading:2521426-Cell Membrane, pubmed-meshheading:2521426-Diglycerides, pubmed-meshheading:2521426-Luminescent Measurements, pubmed-meshheading:2521426-Peptide Fragments, pubmed-meshheading:2521426-Phosphoproteins, pubmed-meshheading:2521426-Phosphorylation, pubmed-meshheading:2521426-Protein Kinase C, pubmed-meshheading:2521426-Second Messenger Systems, pubmed-meshheading:2521426-Tetradecanoylphorbol Acetate, pubmed-meshheading:2521426-Zona Glomerulosa

Effect of ANP on sustained aldosterone secretion stimulated by angiotensin II.

Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't