Linked Life Data

2520825

Source:http://linkedlifedata.com/resource/pubmed/id/2520825

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-7-20
pubmed:abstractText
Ice nucleation activity and the iceC gene product were quantified in different subcellular fractions of the Pseudomonas syringae source strain and in Escherichia coli containing the cloned iceC gene to determine the activity of this protein in different subcellular locations. Ice nuclei were nearly completely retained during isolation of cell envelopes but exhibited a decrease in the temperature at which they were expressed. Ice nucleation activity was found in Triton X-100 insoluble membrane fragments as well as in slowly sedimenting and high-density membrane fragments. Nearly all ice nucleation activity was associated with the outer membrane because the partitioning of 3-ketodeoxyoctonate (a lipopolysaccharide component) and ice nuclei in cell fractions were similar to and opposite that of NADH oxidase (a cytoplasmic membrane component). The iceC gene product had an apparent mass of 150,000 Da based on migration in SDS-polyacrylamide gels. This protein was not found in soluble cell components. Nearly all of the iceC gene product, which occurred in low abundance, was associated with the outer membrane of both P. syringae and E. coli. Therefore, the iceC gene product is located at and is maximally active in or on the outer membrane of cells of the source strain and heterologous strains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0894-0282
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:geneSymbol
iceC
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
262-72
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Localization of ice nucleation activity and the iceC gene product in Pseudomonas syringae and Escherichia coli.
pubmed:affiliation
Department of Plant Pathology, University of California, Berkeley 94720.
pubmed:publicationType
Journal Article