|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1992-2-24
|
|
pubmed:abstractText |
Qualitative and quantitative analysis of human hemoglobin-carcinogen adducts has potential as a diagnostic tool for estimation of biologically effective levels of carcinogen exposure and for attaining a better understanding of individual susceptibility to chemical carcinogenesis. The purpose of this study was to devise a strategy for preanalytical enrichment of the class of covalent human hemoglobin-carcinogen adducts formed by reaction at the hemoglobin beta 93 cysteine sulfhydryl groups. The results define a charge-shift strategy in which a mixture composed of natural hemoglobin (Hb-SH) and low levels of hemoglobin-S-xenobiotic adducts (Hb-SX) is treated with an anionic sulfhydryl reagent (R-), followed by anion-exchange liquid chromatographic separation of Hb-SR- from the unreactive Hb-SX adducts. Using 4-(iodoacetamido)-salicylic acid as the charge-shift reagent, we applied the strategy to the isolation of chromatographically similar adducts with either 4-nitrosobiphenyl or [3H]-N-ethylmaleimide. The strategy was effective for adduct concentrations less than or equal to 10 mumol/mol of hemoglobin. Application of the strategy provides an adduct-enriched fraction useful for subsequent analysis using either currently available techniques or alternate chemical or biochemical techniques that may be designed to take advantage of the enrichment procedure.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-biphenylamine,
http://linkedlifedata.com/resource/pubmed/chemical/4-iodoacetamidosalicylic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Aminobiphenyl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Biphenyl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Ethylmaleimide,
http://linkedlifedata.com/resource/pubmed/chemical/Hemoglobins,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Iodoacetamide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroso Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Salicylic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfhydryl Compounds
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0893-228X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
2
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
379-85
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:2519727-Aminobiphenyl Compounds,
pubmed-meshheading:2519727-Biphenyl Compounds,
pubmed-meshheading:2519727-Carcinogens,
pubmed-meshheading:2519727-Chromatography, Ion Exchange,
pubmed-meshheading:2519727-Cysteine,
pubmed-meshheading:2519727-Erythrocytes,
pubmed-meshheading:2519727-Ethylmaleimide,
pubmed-meshheading:2519727-Hemoglobins,
pubmed-meshheading:2519727-Humans,
pubmed-meshheading:2519727-Hydrolysis,
pubmed-meshheading:2519727-Indicators and Reagents,
pubmed-meshheading:2519727-Iodoacetamide,
pubmed-meshheading:2519727-Nitroso Compounds,
pubmed-meshheading:2519727-Salicylic Acids,
pubmed-meshheading:2519727-Spectrophotometry, Ultraviolet,
pubmed-meshheading:2519727-Sulfhydryl Compounds
|
|
pubmed:articleTitle |
Charge-shift strategy for isolation of hemoglobin-carcinogen adducts formed at the beta 93 cysteine sulfhydryl groups.
|
|
pubmed:affiliation |
Biological and Medical Research Division, Argonne National Laboratory, Illinois 60439-4833.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, Non-P.H.S.
|
