Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0007600,
umls-concept:C0007634,
umls-concept:C0017337,
umls-concept:C0033268,
umls-concept:C0086418,
umls-concept:C0232920,
umls-concept:C0678108,
umls-concept:C1519595,
umls-concept:C1552644,
umls-concept:C1707719,
umls-concept:C1709160,
umls-concept:C1823153,
umls-concept:C2349976
|
| pubmed:issue |
6
|
| pubmed:dateCreated |
1991-5-29
|
| pubmed:abstractText |
A human neoplastic B cell line SSK41 that expresses IgM on its surface switches spontaneously to IgG-producing cells. The SSK41 line contains a single immunoglobulin heavy-chain locus, the constant region (C) genes of which retain the germline configuration. The IgG-producing SSK41 line was purified by sorting, and shown to have undergone S-S recombination with deletion of the C mu gene. This line produced secretory and membrane-bound forms of gamma-chain mRNA. From cDNA libraries of a mixed population of IgM+/IgG+ SSK41 cells, we have isolated cDNA clones encoding the mature membrane-bound and secretory forms of the mu and gamma 1 heavy chains, all of which share the same variable region sequence. cDNA clones containing the mature gamma 3 chain were identified as well. We also isolated cDNA clones containing C gamma 1 and C gamma 3 sterile transcripts from the SSK41 line. These sterile transcripts contained additional exon sequences designated 'I' which were localized upstream of the C gamma 1 and C gamma 3 switch regions and homologous to murine counterparts. The I sequences were precisely spliced to the 5' ends of the corresponding C gamma exon sequences. These features of germline CH transcripts, i.e. the isotype specificity to class switching, location of exons, and sequences per se, are highly conserved between man and mouse.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Constant Regions,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin gamma-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0953-8178
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
631-42
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2518730-Amino Acid Sequence,
pubmed-meshheading:2518730-Animals,
pubmed-meshheading:2518730-B-Lymphocytes,
pubmed-meshheading:2518730-Base Sequence,
pubmed-meshheading:2518730-Genes, Immunoglobulin,
pubmed-meshheading:2518730-Genes, Switch,
pubmed-meshheading:2518730-Humans,
pubmed-meshheading:2518730-Immunoglobulin Constant Regions,
pubmed-meshheading:2518730-Immunoglobulin G,
pubmed-meshheading:2518730-Immunoglobulin M,
pubmed-meshheading:2518730-Immunoglobulin Switch Region,
pubmed-meshheading:2518730-Immunoglobulin gamma-Chains,
pubmed-meshheading:2518730-Mice,
pubmed-meshheading:2518730-Molecular Sequence Data,
pubmed-meshheading:2518730-RNA, Messenger,
pubmed-meshheading:2518730-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2518730-Transcription, Genetic,
pubmed-meshheading:2518730-Tumor Cells, Cultured
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Production of sterile transcripts of C gamma genes in an IgM-producing human neoplastic B cell line that switches to IgG-producing cells.
|
| pubmed:affiliation |
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|