Linked Life Data

2518620

Source:http://linkedlifedata.com/resource/pubmed/id/2518620

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-2-28
pubmed:abstractText
The possible mechanism of the inhibitory effect of endotoxin on adenylate cyclase was investigated. Plasma membranes were isolated from porcine thyroid gland and rat liver. The effects of endotoxin on adenylate cyclase activity was determined. The adenylate cyclase activity followed in the presence of various concentrations of guanosine-imidodiphosphate (5 x 10(-8)-10(-6) M) and NaF (0.1-10 mM) was markedly inhibited by endotoxin. Moreover, the basal activity of adenylate cyclase was inhibited similarly. The inhibition was concentration dependent. At 400 micrograms/ml of endotoxin a 60% inhibition of adenylate cyclase both without and in the presence of activators was detected. The inhibitory effects of endotoxin and its radiodetoxified derivative were similar. Neither of the two investigated endotoxin preparations tested had any effect on the 3H-guanosine-imidodiphosphate binding activity of adenylate cyclase (neither on the binding capacity, nor on the Kd value). Forskolin activated adenylate cyclase was also inhibited markedly by both endotoxin and radiodetoxified endotoxin. These results suggest that beyond the other membrane effects there is a probability of direct inhibitory effect of endotoxin on the catalytic subunit of adenylate cyclase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0237-6261
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
191-202
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
A possible mechanism of the inhibitory effect of endotoxin on adenylate cyclase.
pubmed:affiliation
Frédéric Joliot-Curie National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary.
pubmed:publicationType
Journal Article