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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1990-6-26
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pubmed:abstractText |
We have previously characterized, purified and cloned a novel murine and human regulator [leukaemia inhibitory factor, LIF] which induces the differentiation of certain murine and human myeloid leukaemic cells. Recently we have shown that there are specific LIF receptors on murine embryonic stem [ES] and embryonal carcinoma [EC] cells and that purified recombinant LIF can substitute for feeder cells and crude sources of differentiation inhibiting activity [DIA] [such as BRL-cell-conditioned medium] in the maintenance of ES cells in a pluripotential state in vitro. Furthermore, ES cells maintained in culture in recombinant LIF for a prolonged period can give rise to germline chimaeric mice. Thus, based on a number of biochemical and biological similarities, it is likely that LIF and DIA are the same molecule. The identification of LIF as a molecule, necessary and sufficient for the maintenance of ES cells in culture, should have a profound impact on the use of these cells for genetic manipulations.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1031-3613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
281-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2517664-Amino Acid Sequence,
pubmed-meshheading:2517664-Animals,
pubmed-meshheading:2517664-Cell Differentiation,
pubmed-meshheading:2517664-Cells, Cultured,
pubmed-meshheading:2517664-Embryo, Mammalian,
pubmed-meshheading:2517664-Embryonal Carcinoma Stem Cells,
pubmed-meshheading:2517664-Growth Inhibitors,
pubmed-meshheading:2517664-Humans,
pubmed-meshheading:2517664-Interleukin-6,
pubmed-meshheading:2517664-Leukemia, Myeloid,
pubmed-meshheading:2517664-Leukemia Inhibitory Factor,
pubmed-meshheading:2517664-Lymphokines,
pubmed-meshheading:2517664-Mice,
pubmed-meshheading:2517664-Molecular Sequence Data,
pubmed-meshheading:2517664-Neoplastic Stem Cells,
pubmed-meshheading:2517664-Recombinant Proteins,
pubmed-meshheading:2517664-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2517664-Stem Cells
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pubmed:year |
1989
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pubmed:articleTitle |
LIF: a molecule with divergent actions on myeloid leukaemic cells and embryonic stem cells.
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pubmed:affiliation |
Walter and Eliza Hall Institute for Medical Research, Royal Melbourne Hospital, Parkville, Vic., Australia.
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pubmed:publicationType |
Journal Article,
Review,
Research Support, Non-U.S. Gov't
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