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pubmed-article:2515105pubmed:abstractTextPost-embedding labelling techniques with colloidal gold-IgG or -protein A complexes were used to determine the subcellular location of IFN alpha 1 and staphylokinase secreted from Bacillus subtilis GB500 cells. Both proteins were present in the cytoplasma and the cell envelope pointing to a posttranslational mode of translocation across the cytoplasmic membrane. 5- to 10-fold higher concentrations of gold particles per 0.1 micron 2 were found on the cell envelope and clustering was observed suggesting preferential regions for secretion sites. Several control experiments ensured the specificity of the labelling data.lld:pubmed
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pubmed-article:2515105pubmed:articleTitleImmunoelectron microscopy of Bacillus subtilis cells secreting human interferon alpha 1 or staphylokinase.lld:pubmed
pubmed-article:2515105pubmed:affiliationCentral Institute of Microbiology and Experimental Therapy, Academy of Sciences of G.D.R., Jena, D.D.R.lld:pubmed
pubmed-article:2515105pubmed:publicationTypeJournal Articlelld:pubmed