Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1990-3-14
|
| pubmed:abstractText |
Post-embedding labelling techniques with colloidal gold-IgG or -protein A complexes were used to determine the subcellular location of IFN alpha 1 and staphylokinase secreted from Bacillus subtilis GB500 cells. Both proteins were present in the cytoplasma and the cell envelope pointing to a posttranslational mode of translocation across the cytoplasmic membrane. 5- to 10-fold higher concentrations of gold particles per 0.1 micron 2 were found on the cell envelope and clustering was observed suggesting preferential regions for secretion sites. Several control experiments ensured the specificity of the labelling data.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0378-1097
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
53
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
327-32
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2515105-Bacillus subtilis,
pubmed-meshheading:2515105-Biological Transport,
pubmed-meshheading:2515105-Humans,
pubmed-meshheading:2515105-Immunohistochemistry,
pubmed-meshheading:2515105-Interferon Type I,
pubmed-meshheading:2515105-Metalloendopeptidases,
pubmed-meshheading:2515105-Microscopy, Electron
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Immunoelectron microscopy of Bacillus subtilis cells secreting human interferon alpha 1 or staphylokinase.
|
| pubmed:affiliation |
Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of G.D.R., Jena, D.D.R.
|
| pubmed:publicationType |
Journal Article
|