Linked Life Data

2514352

Source:http://linkedlifedata.com/resource/pubmed/id/2514352

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1990-2-22
pubmed:abstractText
During the course of B lymphocyte differentiation into immunoglobulin secreting cells the abundance of mRNA for the heavy chain of secreted IgM (microS) increases dramatically. In order to understand the regulatory events responsible for the selective increase in micS mRNA we have looked for transcriptional alterations of VDJC mu gene segments as well as changes in the relative stability of microM and microS mRNA in BCL1 lymphoma cells which can be stimulated to increase the expression of microS mRNA. These experiments showed that although the transcriptional level of the mu gene locus is not preferentially augmented after stimulation, an alteration in the sites of polymerase termination is a significant factor contributing to the higher microS to microM ratio. This switch is dependent on new RNA synthesis. In addition, although the half-life of microS mRNA is not selectively increased, stimulation of the cells does result in a specific enhancement of the half-lives of both species of mu mRNA, which accounts for the higher steady state levels of total mu message.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1059-67
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Regulation of microM vs microS mRNA expression in an inducible B cell line.
pubmed:affiliation
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.