Linked Life Data

2513388

Source:http://linkedlifedata.com/resource/pubmed/id/2513388

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-2-8
pubmed:abstractText
Hamster zonae pellucidae were labelled with fluorescein isothiocyanate and placed under thin polyacrylamide sheets polymerized on the floor of an observation chamber. The integrity of these FITC-labelled zonae pellucidae was monitored continuously by fluorescence microscopy using a silicon-intensified target video-camera during exposure to a 0.1% sodium dodecyl sulphate solution diffusing through the polyacrylamide sheet. Complete dissolution of zonae pellucidae was observed at the threshold level of detection (estimated as 0.2% of the signal from a labelled zona pellucida). Similar behaviour was shown by zonae pellucidae isolated from hamster eggs 5, 15 and 30 min after a cortical reaction induced by the ionophore A23187. These findings suggest that the hamster zona pellucida contains no underlying covalent matrix before the cortical reaction, and that the cortical reaction does not produce covalent inter-molecular cross-linking of the zona pellucida.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-4251
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
447-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Effect of sodium dodecyl sulphate on the hamster zona pellucida before and after a cortical reaction induced by the ionophore A23187.
pubmed:affiliation
Department of Pharmacology, University of Otago Medical School, Dunedin, New Zealand.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't