Linked Life Data

2512769

Source:http://linkedlifedata.com/resource/pubmed/id/2512769

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-1-12
pubmed:abstractText
Carbonic anhydrase (CA) isozymes CA I and CA II were isolated from rat erythrocytes, and CA III from rat skeletal muscle. They were purified to homogeneity and labelled with 125I using the Bolton-Hunter method. The tissue distribution of these [125I]CA isozymes was studied in rats with whole-body autoradiography at various times after an intravenous injection. The distribution pattern showed a remarkable organ specificity. CA I and CA III were to a great extent localized to the renal cortex. This is compatible with renal uptake, secondary to glomerular filtration, of these isozymes. This would be expected from the renal handling of proteins with the following characteristics: molecular weight of 29,000; iso-electric points, pI, around 7.2 and 6.5 respectively. However, CA II of similar molecular shape and size, with a pI of 6.8, remained in the blood and was preferentially localized to the liver. Further studies are needed to clarify why such similar proteins are targeted to different organs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0001-6772
pubmed:author
pubmed:issnType
Print
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
449-56
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Tissue distribution of 125I-labelled carbonic anhydrase isozymes I, II and III in the rat.
pubmed:affiliation
Department of Pharmacology and Toxicology, Swedish University of Agricultural Sciences, Uppsala.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't