Linked Life Data

2512260

Source:http://linkedlifedata.com/resource/pubmed/id/2512260

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-1-24
pubmed:abstractText
The C57Bl/6-derived T cell line, L12-R4, produced murine interferon-gamma (IFN gamma) in response to mitogenic stimulation by phorbol myristate acetate (PMA) or concanavalin A (Con A), but not by staphylococcal enterotoxin A (SEA). Low levels of IFN gamma were produced by SEA stimulation of L12-R4 cells cocultured with C57Bl/6 bone marrow macrophages (BMM). Significantly increased yields of IFN gamma resulted from 48-hour pretreatment of the BMM with recombinant IFN gamma (100 U/ml) prior to coculture. Polyclonal anti-IFN gamma and anti-IFN alpha/beta were used to characterize the interferon as IFN gamma. Paraformaldehyde (0.1%) treatment of IFN gamma-pretreated BMM did not affect IFN gamma production, suggesting that processing of SEA was not required. IFN gamma treatment of BMM resulted in significantly increased expression of immune-associated (Ia) antigen as determined by flow cytometric analysis, suggesting that the accessory cell role of BMM involved Ia antigen. Polyclonal anti-Ia antibody selectively inhibited the production of IFN gamma by SEA-stimulated whole spleen cell cultures, consistent with the necessity of Ia antigen for BMM help in SEA induction of IFN gamma. More interestingly, induction of IFN gamma. These findings suggest that Ia antigen is necessary for BMM accessory function in SEA induction of IFN gamma. More interestingly, the results implicate class II molecules in a positive feedback loop for IFN gamma production by SEA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0020-5915
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
219-23
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
A positive feedback loop for staphylococcal enterotoxin-A-stimulated IFN-gamma production requires macrophage immune-associated antigen upregulation.
pubmed:affiliation
Department of Microbiology and Cell Science, University of Florida, Gainesville.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.