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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1990-1-8
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pubmed:abstractText |
A protein that binds to the recombination signal sequence (RS) of the immunoglobulin J kappa segment was purified almost to homogeneity from the nuclear extract of a murine pre-B cell line 38B9. A similar binding protein was found in lymphoid cell lines but not in non-lymphoid cell lines. The binding activity was associated with a polypeptide with a molecular weight of 60,000. DNase I footprinting analysis demonstrated that this binding protein interacted with the heptamer and several 3' bases close to the heptamer. The Kd value of the J kappa RS binding protein to the J kappa RS was 1 nM. One base substitution in the heptamer of the J kappa RS greatly reduced the affinity of the J kappa RS binding protein. The high specificity of the binding site of the J kappa RS binding protein suggests that this protein may be involved in V-J recombination.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-111144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-111146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-14056989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-2562155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3079885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3118358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3120312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3158075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3484682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3495343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3500258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3500792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3924409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-3927822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-4075400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-6277505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-6300689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-6323991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-6327046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-6443559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-6611508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-6769593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-6774258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2511556-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9015-26
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2511556-Animals,
pubmed-meshheading:2511556-B-Lymphocytes,
pubmed-meshheading:2511556-Base Sequence,
pubmed-meshheading:2511556-Cell Line,
pubmed-meshheading:2511556-Cell Nucleus,
pubmed-meshheading:2511556-Chromatography, Affinity,
pubmed-meshheading:2511556-DNA Probes,
pubmed-meshheading:2511556-DNA-Binding Proteins,
pubmed-meshheading:2511556-Deoxyribonuclease I,
pubmed-meshheading:2511556-Genes, Immunoglobulin,
pubmed-meshheading:2511556-Immunoglobulin J-Chains,
pubmed-meshheading:2511556-Immunoglobulin kappa-Chains,
pubmed-meshheading:2511556-Kinetics,
pubmed-meshheading:2511556-Mice,
pubmed-meshheading:2511556-Molecular Sequence Data,
pubmed-meshheading:2511556-Protein Sorting Signals,
pubmed-meshheading:2511556-Recombination, Genetic
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pubmed:year |
1989
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pubmed:articleTitle |
Purification and characterization of a protein that binds to the recombination signal sequence of the immunoglobulin J kappa segment.
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pubmed:affiliation |
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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