Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1989-10-31
pubmed:abstractText
A gene from Bacillus thuringiensis subsp. kurstaki that codes for a Lepidoptera-specific insecticidal toxin (delta-endotoxin) was engineered for expression in Bacillus subtilis. A low-copy-number plasmid vector that replicates in Escherichia coli and B. subtilis was constructed to transform B. subtilis with gene fusions first isolated and characterized in E. coli. Naturally occurring promoter sequences from B. subtilis (43, veg, ctc, and spoVG) were inserted upstream from the plasmid-borne structural gene. In the most prolific case, when the sporulation-specific spoVG promoter was fused to the heterologous toxin gene, the toxin product accumulated during postexponential growth to greater than 25% of the total cell protein. However, the resulting specific activity of the insecticidal toxin product was not commensurate with the abundance of the protein.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-16561900, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-2541663, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-3007434, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-3007957, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-3011746, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-3099284, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-3102457, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-3277943, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-344137, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-3535652, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-404641, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-408013, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-4896022, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-4922220, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-4937299, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-4956288, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-6181373, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-6202876, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-6268988, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-6296116, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-6304019, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-6329704, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-6414720, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-6793556, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-6793593, http://linkedlifedata.com/resource/pubmed/commentcorrection/2508556-7029580
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2302-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Accumulation of the insecticidal crystal protein of Bacillus thuringiensis subsp. kurstaki in post-exponential-phase Bacillus subtilis.
pubmed:affiliation
Syntro Corporation, San Diego, California 92121.
pubmed:publicationType
Journal Article